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NSE Mouse mAb - Primary antibody, specific to NSE, Mouse IgG2b

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: C10
  • Conjugation: Unconjugated
In stock
Item Number
Ab118776
Grouped product items
SKU Size
Availability
 Price Qty
Ab118776-10μl
10μl
5
$66.90
Ab118776-50μl
50μl
5
$189.90
Ab118776-100μl
100μl
1
$279.90
Ab118776-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (C10); Mouse anti Human NSE Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: ENO2
Accession#:P09104 (3) ENO2 (3) Gene ID:2026 (3) NSE (3)

Basic Description

Product Name NSE Mouse mAb - Primary antibody, specific to NSE, Mouse IgG2b
Synonyms 2-phospho-D-glycerate hydro-lyase | EC 4.2.1.11 | Enolase 2 | Neural enolase | Neuron-specific enolase | NSE
Specifications & Purity Carrier Free, ExactAb™, Validated, See COA
Host species Mouse
Specificity NSE
Immunogen Recombinant NSE expressed in E.coli (AA 2-285)
Positive Control WB: Hep G2, NIH/3T3, C6 and Jurkat cell lysates. IHC: Human Glioma Tissue, Human Cerebrum Tissue. IF/ICC: Hep G2 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human NSE Antibody, Monoclonal (C10), could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 0.5-2µg/mL
IHC: 5-30µg/mL
ICC/IF: 5-30µg/mL
Protein Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Product Properties

Isotype Mouse IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A + Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ENO2 Tbio Gamma-enolase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

NSE Mouse mAb (Ab118776) - Western Blot
All lanes: NSE Mouse mAb (Ab118776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 16.4 s

NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Glioma Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

NSE Mouse mAb (Ab118776) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 1µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application

Application Dilution info
IHC

5-30 µg/mL
WB

0.5-2 µg/mL
IF/ICC

5-30 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901047 Certificate of Analysis Sep 18, 2023 Ab118776
ZJ23F0901046 Certificate of Analysis Sep 18, 2023 Ab118776

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