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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab117335-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab117335-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab117335-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab117335-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
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pAb; Rabbit anti Human NDUFA4 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | NDUFA4 Antibody - Primary antibody, specific to NDUFA4, Rabbit IgG |
|---|---|
| Synonyms | CI 9k | CI-MLRQ | Complex I 9kDa subunit | Complex I-MLRQ | MLRQ | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 9kD | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 | NADH-ubiquinone oxidoreductase MLRQ subunit | NDUA4_HUMAN | NDUFA4 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron |
| Host species | Rabbit |
| Specificity | NDUFA4 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NDUFA4 |
| Positive Control | WB: Mouse kidney, Rat kidney, HeLa, Hep G2 and THP-1 lysates. IHC:Human brain tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human NDUFA4 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
NDUFA4 Antibody (Ab117335) - Western Blot
All lanes: NDUFA4 Antibody (Ab117335) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 1.9 s
NDUFA4 Antibody (Ab117335) - Western Blot
All lanes: NDUFA4 Antibody (Ab117335) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 1.9 s
NDUFA4 Antibody (Ab117335) - IHC
Immunohistochemical analysis of NDUFA4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with NDUFA4 Antibody (Ab117335) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
NDUFA4 Antibody (Ab117335) - IF/ICC
Immunofluorescent analysis of NDUFA4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with NDUFA4 Antibody (Ab117335) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| WB | 1/500-1/1000 |
| IF/ICC |
1/100-1/500
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