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NDUFA4 Antibody - Primary antibody, specific to NDUFA4, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab117335
Grouped product items
SKU Size
Availability
Price Qty
Ab117335-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab117335-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab117335-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab117335-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

pAb; Rabbit anti Human NDUFA4 Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product Name NDUFA4 Antibody - Primary antibody, specific to NDUFA4, Rabbit IgG
Synonyms CI 9k | CI-MLRQ | Complex I 9kDa subunit | Complex I-MLRQ | MLRQ | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 9kD | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 | NADH-ubiquinone oxidoreductase MLRQ subunit | NDUA4_HUMAN | NDUFA4
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron
Host species Rabbit
Specificity NDUFA4
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NDUFA4
Positive Control WB: Mouse kidney, Rat kidney, HeLa, Hep G2 and THP-1 lysates. IHC:Human brain tissue. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human NDUFA4 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

NDUFA4 Tclin Cytochrome c oxidase subunit NDUFA4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

NDUFA4 Antibody (Ab117335) - Western Blot
All lanes: NDUFA4 Antibody (Ab117335) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 1.9 s

NDUFA4 Antibody (Ab117335) - Western Blot
All lanes: NDUFA4 Antibody (Ab117335) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 1.9 s

NDUFA4 Antibody (Ab117335) - IHC
Immunohistochemical analysis of NDUFA4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with NDUFA4 Antibody (Ab117335) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

NDUFA4 Antibody (Ab117335) - IF/ICC
Immunofluorescent analysis of NDUFA4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with NDUFA4 Antibody (Ab117335) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Application

Application Dilution info
IHC

1/100 - 1/200

WB

1/500-1/1000

IF/ICC

1/100-1/500

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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