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MTHFD2 Mouse mAb - Primary antibody, specific to MTHFD2, Mouse IgG1

    Application:
  • IHC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: K70015_2H12
  • Conjugation: Unconjugated
In stock
Item Number
Ab116276
Grouped product items
SKU Size
Availability
Price Qty
Ab116276-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab116276-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab116276-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab116276-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

mAb (K70015_2H12); Mouse anti Human MTHFD2 Antibody; WB, IHC, IP; Unconjugated

Basic Description

Product Name MTHFD2 Mouse mAb - Primary antibody, specific to MTHFD2, Mouse IgG1
Synonyms NMDMC | MTHFD2 | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody | Descriptions antibody | Methenyltetrahydrofolate cyclohydrolase antibody | methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody |
Specifications & Purity Carrier Free, ExactAb™, Validated, 1.0 mg/mL
Host species Mouse
Specificity MTHFD2
Immunogen Recombinant protein of human MTHFD2 (AA 101-300)
Positive Control WB: A431, Hep G2, HeLa, HEK293 and MOLT-4 cell lysates. IHC: Human kidney tissue. IP: A431 lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human MTHFD2 Antibody, Monoclonal (K70015_2H12), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/200
IP:1/100

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MTHFD2 Tchem Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

MTHFD2 Mouse mAb (Ab116276) - Western Blot
All lanes: MTHFD2 Mouse mAb (Ab116276) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 34 kDa
Exposure time: 169.7 s

MTHFD2 Mouse mAb (Ab116276) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of MTHFD2 Mouse mAb (Ab116276) with A431 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MTHFD2Mouse mAb (Ab116276) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: A431 lysateLane 2: MTHFD2 immunoprecipitated from A431 lysate by MTHFD2 Mouse mAb (Ab116276
Lane 3: IgG isotype control antibody instead of MTHFD2 Mouse mAb (Ab116276) in A431 lysate.
Result: MTHFD2 Mouse mAb (Ab116276) can immunoprecipitate MTHFD2.

MTHFD2 Mouse mAb (Ab116276) - IHC
IHC-P analysis of kidney tissue by MTHFD2 Mouse mAb (Ab116276).IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with MTHFD2 Mouse mAb (Ab116276) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in tubules are positively stained at the cytoplasm.

Application

Application Dilution info
WB

1/1000

IP

1/100

IHC

1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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