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MEK4/MKK4 Antibody - Primary antibody, specific to MAP2K4, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Monkey,Mouse,Rat,Zebrafish
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab114888
Grouped product items
SKU Size
Availability
 Price Qty
Ab114888-10μl
10μl
1
$49.90
Ab114888-50μl
50μl
1
$149.90
Ab114888-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab114888-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Rabbit anti Human MEK4/MKK4 Antibody; WB, IHC, IF/ICC; Unconjugated

View related series
Click to view target information: MAP2K4
Accession#: P45985 (1) Gene ID: 6416 (1) MAP2K4 (1) MEK4 (1) MKK4 (1)

Basic Description

Product Name MEK4/MKK4 Antibody - Primary antibody, specific to MAP2K4, Rabbit IgG
Synonyms c-Jun N-terminal kinase kinase 1 | dual specificity mitogen-activated protein kinase kinase 4 | JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | JNKK | JNKK1 | JNKK1MAPK/ERK kinase 4 | MAP kinase kinase 4 | MAP2K4 | MAPKK4 | ME
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Rabbit
Specificity MAP2K4
Immunogen A KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MKK4
Positive Control WB: HeLa, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human skeletal muscle tissue. IF/ICC: COS7 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human MEK4/MKK4 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on.
Application：
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function：
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MAP2K4 Tchem Dual specificity mitogen-activated protein kinase kinase 4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

MEK4/MKK4 Antibody (Ab114888) - Western Blot
All lanes: MEK4/MKK4 Antibody (Ab114888) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 46 kDa
Exposure time: 17.4 s

MEK4/MKK4 Antibody (Ab114888) - IF/ICC
Immunofluorescent analysis of MEK4/MKK4 Antibody (Ab114888) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton
X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS
for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

MEK4/MKK4 Antibody (Ab114888) - IHC
Immunohistochemical analysis of MEK4/MKK4 Antibody (Ab114888) staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

Application Dilution info
IF/ICC

1/50 - 1/200
WB

1/500-1/1000

IHC

1/50-1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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