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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab113719-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
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Ab113719-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$189.90
|
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Ab113719-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab113719-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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Recombinant; Rabbit anti Human Lunatic Fringe Antibody; WB, IF/ICC; Unconjugated
| Product Name | Recombinant Lunatic Fringe Antibody - Primary antibody, specific to LFNG, Rabbit IgG |
|---|---|
| Synonyms | EC 2.4.1.222 | O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segm |
| Host species | Rabbit |
| Specificity | LFNG |
| Immunogen | A synthetic peptide derived from human LFNG (AA 350-379) |
| Positive Control | WB: HeLa, K562, HT-29, MCF7 and NIH/3T3 cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Lunatic Fringe Antibody, Recombinant, could be used for WB, IF/ICC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant Lunatic Fringe Antibody (Ab113719) - Western Blot
All lanes: Recombinant Lunatic Fringe Antibody (Ab113719) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 46 kDa
Lunatic Fringe has 4 isoforms produced by alternative splicing with molecular weights of 28, 35, 40 and 42 kDa.
Exposure time: 0.1 s
Recombinant Lunatic Fringe Antibody (Ab113719) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant Lunatic Fringe Antibody (Ab113719) at 1/50 dilution.
Recombinant Lunatic Fringe Antibody (Ab113719) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant Lunatic Fringe Antibody (Ab113719) at 1/150 dilution.
Recombinant Lunatic Fringe Antibody (Ab113719) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant Lunatic Fringe Antibody (Ab113719) at 1/500 dilution.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
|
| WB | 1/1000 - 1/2000 |