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Immunology
IL-33 Mouse mAb - Primary antibody, specific to IL33, Mouse IgG2a

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IL-33 Mouse mAb - Primary antibody, specific to IL33, Mouse IgG2a

Grade & Purity:

High Performance

See COA

Application:

IHC
WB

Host species: Mouse
Reactive species: Human,Mouse,Rat
Isotype: Mouse IgG2a
Clone Number: C11
Conjugation: Unconjugated

In stock

Item Number

Ab006917

Grouped product items
SKU	Size	Availability	Price	Qty
Ab006917-10μl	10μl	≥10	$66.90
Ab006917-50μl	50μl	7	$189.90
Ab006917-100μl	100μl	4	$299.90
Ab006917-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,299.90

mAb (C11); Mouse anti Human IL-33 Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: IL33

Accession#:O95760 (6) Gene ID:90865 (6) IL33 (6)

Basic Description

Product Name	IL-33 Mouse mAb - Primary antibody, specific to IL33, Mouse IgG2a
Synonyms	IL-1F11 \| IL-33 \| Interleukin-1 family member 11 \| NF-HEV \| Nuclear factor from high endothelial venules
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species	Mouse
Specificity	IL33
Immunogen	Recombinant IL33 expressed in E.coli (AA 112-270).
Positive Control	WB: Jurkat, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human Stomach cancer Tissue, Human Prostate cancer Tissue, Human Colorectal cancer Tissue, Human Breast cancer Tissue, Human Liver Tissue.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description	Mouse anti Human IL-33 Antibody, Monoclonal antibody (C11), could be used for WB, IHC and so on. Application WB: 0.01-3µg/mL IHC: 5-30µg/mL Protein Function Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.

Product Properties

Isotype	Mouse IgG2a
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A + Protein G affinity chromatography
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

IL33 Tbio Interleukin-33 (0 Activities)

Discover Target: IL33

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - Western Blot
All lanes: IL-33 Mouse mAb (Ab006917) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 150.0 s

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Stomach cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application

Application	Dilution info
IHC	5-30 µg/mL
WB	0.01-3 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102402	Certificate of Analysis	Jan 10, 2024	Ab006917
ZJ24F0102401	Certificate of Analysis	Jan 10, 2024	Ab006917
ZJ24F0102400	Certificate of Analysis	Jan 10, 2024	Ab006917

Documents & Articles

Immunohistochemical staining protocol for frozen samples (IHC-Fr)

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies

Immunohistochemistry (IHC) staining antigen retrieval protocol

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval

Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol

Categories: Protocols

Tags:

Cytokines Growth factors ELISPOT Secreted Proteins HRP Conjugated Secondary Antibodies

Sodium Azide Removal Protocol

Categories: Protocols

Tags:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

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