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ENO1 Mouse mAb - Primary antibody, specific to ENO1, Mouse IgG1

COA COA
    Application:
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Cow,Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: C2
  • Conjugation: Unconjugated
In stock
Item Number
Ab101688
Grouped product items
SKU Size
Availability
 Price Qty
Ab101688-10μl
10μl
1
$59.90
Ab101688-50μl
50μl
4
$179.90
Ab101688-100μl
100μl
3
$279.90
Ab101688-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (C2); Mouse anti Human ENO1 Antibody; WB, IHC; Unconjugated

View related series
Click to view target information: ENO1
Accession#:P06733 (2) ENO1 (2) Gene ID:2023 (2)

Basic Description

Product Name ENO1 Mouse mAb - Primary antibody, specific to ENO1, Mouse IgG1
Synonyms 2 phospho D glycerate hydro lyase antibody | 2-phospho-D-glycerate hydro-lyase antibody | Alpha enolase antibody | Alpha enolase like 1 antibody | Alpha-enolase antibody | C myc promoter binding protein antibody | C-myc promoter-binding protein antibody |
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species Mouse
Specificity ENO1
Immunogen Recombinant ENO1 expressed in E.coli (AA 1-434)
Positive Control WB: HeLa, MCF7, A549, THP-1, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue.
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human ENO1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-20μg/mL

Protein Function

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A + Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ENO1 Tchem Alpha-enolase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

ENO1 Mouse mAb (Ab101688) - Western Blot
All lanes: ENO1 Mouse mAb (Ab101688) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 48 kDa
Exposure time: 2.5 s

ENO1 Mouse mAb (Ab101688) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

ENO1 Mouse mAb (Ab101688) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application

Application Dilution info
IHC

5-20 µg/mL
WB

0.01-2 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101820 Certificate of Analysis Nov 27, 2023 Ab101688
ZJ23F1101817 Certificate of Analysis Nov 27, 2023 Ab101688

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