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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab190207-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$10.90
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Ab190207-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$20.90
|
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Ab190207-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$59.90
|
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mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (Biotin); Flow
| Product Name | CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2a |
|---|---|
| Synonyms | Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | Biotin Anti-Mouse CD8a (53-6.7) | Biotin Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), Biotin | Biotin anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibo |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains |
| Host species | Rat |
| Specificity | CD8 alpha |
| Immunogen | Mouse Spleen Cells or Thymocyte Membranes |
| Positive Control | Flow: C57BL/6 mouse splenocytes. |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.
|
| Isotype | Rat IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD8 alpha Rat mAb (Biotin) (Ab190207) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab190207) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).
CD8 alpha Rat mAb (Biotin) (Ab190207) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab190207) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry |
≤0.3 μg/test,
the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume
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