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CD8 alpha Rat mAb (PE-Cy5) - Primary antibody, specific to CD8 alpha

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: 53-6.7
  • Conjugation: PE-Cy5
In stock
Item Number
Ab190210
Grouped product items
SKU Size
Availability
Price Qty
Ab190210-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$36.90
Ab190210-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$72.90
Ab190210-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90

mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (PE-Cy5); Flow Cytometry

Basic Description

Product Name CD8 alpha Rat mAb (PE-Cy5) - Primary antibody, specific to CD8 alpha
Synonyms Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Host species Rat
Specificity CD8 alpha
Immunogen Mouse Spleen Cells or Thymocyte Membranes
Positive Control Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation PE-Cy5
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 670nm
Ex/Em(nm) Ex:565nm;Em:670nm
Grade ExactAb™, Validated
Product Description

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD8 alpha Rat mAb (PE-Cy5) (Ab190210) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy5) (Ab190210) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy5) (Ab190072).

CD8 alpha Rat mAb (PE-Cy5) (Ab190210) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy5) (Ab190210) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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