Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab190210-10μg
|
10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$36.90
|
|
|
Ab190210-25μg
|
25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$72.90
|
|
|
Ab190210-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$189.90
|
|
mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (PE-Cy5); Flow Cytometry
| Product Name | CD8 alpha Rat mAb (PE-Cy5) - Primary antibody, specific to CD8 alpha |
|---|---|
| Synonyms | Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| Host species | Rat |
| Specificity | CD8 alpha |
| Immunogen | Mouse Spleen Cells or Thymocyte Membranes |
| Positive Control | Flow Cytometry: C57BL/6 mouse splenocytes |
| Conjugation | PE-Cy5 |
| Excitation(nm) | Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm) |
| Emission(nm) | 670nm |
| Ex/Em(nm) | Ex:565nm;Em:670nm |
| Grade | ExactAb™, Validated |
| Product Description |
Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD8 alpha Rat mAb (PE-Cy5) (Ab190210) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy5) (Ab190210) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy5) (Ab190072).
CD8 alpha Rat mAb (PE-Cy5) (Ab190210) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy5) (Ab190210) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
Starting at $13.90
Starting at $59.90
Starting at $16.90
Starting at $29.90
Starting at $29.90
Starting at $11.90
Starting at $19.90
Starting at $29.90
Starting at $29.90
Starting at $10.90
Starting at $47.90
Starting at $31.90
Starting at $11.90
Starting at $29.90
Starting at $19.90
Starting at $11.90
Starting at $10.90