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Recombinant CD8 alpha Antibody (PE) - Primary antibody, specific to Mouse CD8α

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: P83E51
  • Conjugation: PE
In stock
Item Number
Ab213872
Grouped product items
SKU Size
Availability
Price Qty
Ab213872-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$11.90
Ab213872-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
Ab213872-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$54.90

Recombinant (P83E51); Rat anti Mouse CD8α Antibody (PE); Flow Cytometry

Basic Description

Product Name Recombinant CD8 alpha Antibody (PE) - Primary antibody, specific to Mouse CD8α
Synonyms alpha polypeptide (p32) antibody | CD_antigen=CD8a antibody | CD8 antibody | CD8 antigen alpha polypeptide antibody | CD8 antigen alpha polypeptide (p32) antibody | CD8 antigen, alpha polypeptide (p32) antibody | CD8a antibody | CD8A antigen antibody | CD
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com
Host species Rat
Specificity Mouse CD8α
Immunogen Mouse Spleen Cells or Thymocyte Membranes
Positive Control Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade ExactAb™, Validated
Product Description

Recombinant CD8α Antibody, clone P83E51, could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant CD8 alpha Antibody (PE) (Ab213872) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD8 alpha Antibody (PE) (Ab213872) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Recombinant CD8 alpha Antibody (PE) (Ab213872) - Flow Cytometry
Flow cytometry analysis of CD8 alpha by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD8 alpha Antibody (PE) (Ab213872) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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