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Rat IgG (Biotin) - Isotype Control Antibody, IgG

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Isotype: Rat IgG
  • Conjugation: Biotin
In stock
Item Number
Ab180310
Grouped product items
SKU Size
Availability
 Price Qty
Ab180310-10μg
10μg
≥10
$19.90
Ab180310-100μg
100μg
6
$28.90
Ab180310-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Isotype Control Antibody; Rat IgG (Biotin); Flow, ELISA

View related series
Flow Cytometry (1158) IgG (55) Isotype Control Antibody (126) Rat IgG (16)

Basic Description

Product Name Rat IgG (Biotin) - Isotype Control Antibody, IgG
Synonyms Rat IgG | Biotin-conjugated Rat Polyclonal antibody | Biotin Rat IgG - Isotype Control
Specifications & Purity ExactAb™, Validated, Azide Free, 0.5 mg/mL
Host species Rat
Conjugation Biotin
Grade Azide Free, ExactAb™, Validated
Product Description

Application:

Flow: ≤1μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

Isotype IgG
SDS-PAGE 150 kDa
Purification Method Affinity chromatography
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - ELISA
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was tested by direct ELISA. The plate was coated with Goat Anti-Rat IgG H&L Antibody (Ab138352). A serial dilution of Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD16/CD32 Rat mAb (Biotin) (Ab179681) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0303390 Certificate of Analysis Mar 19, 2024 Ab180310
ZJ24F0303391 Certificate of Analysis Mar 19, 2024 Ab180310
ZJ24R0200271 Certificate of Analysis Feb 02, 2024 Ab180310

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