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CD8 alpha Rat mAb (PE) - Primary antibody, specific to CD8 alpha

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: 53-6.7
  • Conjugation: PE
In stock
Item Number
Ab190209
Grouped product items
SKU Size
Availability
 Price Qty
Ab190209-10μg
10μg
2
$11.90
Ab190209-25μg
25μg
2
$21.90
Ab190209-100μg
100μg
1
$59.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (PE); Flow Cytometry

View related series
Click to view target information: CD8A
Accession#:P01731 (20) Flow Cytometry (1158) Gene ID:12525 (20) Ly-2 (14) Ly-35 (13) Ly-B (13) Lyt-2 (16) PE (167)

Basic Description

Product Name CD8 alpha Rat mAb (PE) - Primary antibody, specific to CD8 alpha
Synonyms Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | PE Anti-Mouse CD8a (53-6.7) | PE Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), PE | PE anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibody | T8 T cell a
Specifications & Purity ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL
Biochemical and Physiological Mechanisms Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Host species Rat
Specificity CD8 alpha
Immunogen Mouse Spleen Cells or Thymocyte Membranes
Positive Control Flow: C57BL/6 mouse splenocytes.
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade Em:575nm, Ex:565nm, ExactAb™, Validated
Product Description

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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