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CD105 Mouse mAb - Primary antibody, specific to CD105, IgG1

    Application:
  • ELISA
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: 3A9
  • Conjugation: Unconjugated
In stock
Item Number
Ab093671
Grouped product items
SKU Size
Availability
Price Qty
Ab093671-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab093671-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$200.90
Ab093671-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$309.90
Ab093671-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,624.90

mAb (3A9); Mouse anti Human CD105 Antibody; WB, IHC, Flow, ELISA, ICC, IF; Unconjugated

Basic Description

Product Name CD105 Mouse mAb - Primary antibody, specific to CD105, IgG1
Synonyms AI528660 antibody | AI662476 antibody | CD 105 antibody | CD105 antibody | CD105 antigen antibody | EGLN_HUMAN antibody | END antibody | Endoglin antibody | Eng antibody | FLJ41744 antibody | HHT1 antibody | ORW antibody | ORW1 antibody | Osler Rendu Webe
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Mouse
Specificity CD105
Immunogen Purified recombinant fragment of Human CD105, expressed in E. coli
Positive Control WB: HUVEC cell lysate. ICC/IF: Hep G2 cells. Flow: Hep G2 cells. IHC: Human kidney cancer and stomach cancer tissues.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse monoclonal (3A9) to CD105, could be used for WB, IHC, Flow, ICC, IF and ELISA.
Application:
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC/IF: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function:
ENG encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia,also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. 

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles.

Associated Targets(Human)

ENG Tbio Endoglin (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD105 Mouse mAb (Ab093671) - Western Blot
All lanes: CD105 Mouse mAb (Ab093671) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 71 kDa
Observed band size: 93 kDa

CD105 Mouse mAb (Ab093671) - ICC/IF
Immunofluorescence analysis of Hep G2 cells using CD105 Mouse mAb (Ab093671) at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.

CD105 Mouse mAb (Ab093671) - Flow Cytometry
Flow cytometric analysis of Hep G2 cells using CD105 Mouse mAb (Ab093671)
at a dilution of 1/200 (green) and negative control (purple).

CD105 Mouse mAb (Ab093671) - IHC
Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissues using CD105 Mouse mAb (Ab093671) at a dilution of 1/200 with DAB staining.

CD105 Mouse mAb (Ab093671) - IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using CD105 Mouse mAb (Ab093671) at a dilution of 1/200 with DAB staining.

Application

Application Dilution info
WB

1/500-1/2000

IF/ICC

1/200 - 1/1000

ELISA

1/10000

IHC

1/200-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800758 Certificate of Analysis Aug 03, 2023 Ab093671
ZJ23F0800759 Certificate of Analysis Aug 03, 2023 Ab093671

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