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Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: FG01/2C1
  • Conjugation: Unconjugated
In stock
Item Number
Ab188096
Grouped product items
SKU Size
Availability
 Price Qty
Ab188096-10μl
10μl
≥10
$69.90
Ab188096-50μl
50μl
7
$199.90
Ab188096-100μl
100μl
2
$319.90
Ab188096-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (FG01/2C1); Mouse anti Human Smad3 Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: SMAD3
Accession#:P84022 (4) Flow Cytometry (1158) Gene ID:4088 (6) SMAD3 (3)

Basic Description

Product Name Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b
Synonyms DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
Specifications & Purity Validated, ExactAb™, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Host species Mouse
Specificity Smad3
Immunogen Recombinant full length protein corresponding to Human Smad3
Positive Control WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells ICC/IF: U-2 OS cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

WB: 1/1000 – 1/100,000

Flow: 1/200 - 1/2,000

ICC/IF: 1/200

Product Properties

Isotype Mouse IgG2b
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 49 kDa
Exposure time: 0.9 s 

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 29.6 s 

Samd3 Mouse mAb (Ab188096) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Samd3 Mouse mAb (Ab188096) - ICC/IF
IF analysis of Samd3 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Samd3 Mouse mAb (Ab188096) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
Flow Cytometry

1/200-1/2000
IF/ICC

1/200

WB

1/1000 – 1/100,000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0607493 Certificate of Analysis Jun 27, 2024 Ab188096
ZJ24F0607492 Certificate of Analysis Jun 27, 2024 Ab188096
ZJ24F0607491 Certificate of Analysis Jun 27, 2024 Ab188096
ZJ24R0600677 Certificate of Analysis Jun 14, 2024 Ab188096

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