Grouped product items

SKU

Size

Availability

Price

Qty

Ab114851-10μl

10μl

Available within 8-12 weeks(?)

$69.90

Ab114851-50μl

50μl

$199.90

Ab114851-100μl

100μl

Available within 8-12 weeks(?)

$319.90

Ab114851-1ml

1ml

Available within 8-12 weeks(?)

$2,099.90

Basic Description

Product Name	Recombinant MEK1/MEK2 Antibody - Primary antibody, specific to MEK1/MEK2, Rabbit IgG
Synonyms	AA589381 antibody \| CFC3 antibody \| Dual specificity mitogen-activated protein kinase kinase 1 antibody \| Dual specificity mitogen-activated protein kinase kinase 2 antibody \| EC 2.7.12.2 antibody \| ERK activator kinase 1 antibody \| ERK activator kinase 2
Specifications & Purity	Recombinant, ExactAb™, Validated, High Performance, See COA
Host species	Rabbit
Specificity	MEK1/MEK2
Immunogen	A synthetic peptide derived from human MEK1/MEK2 (AA 1-393).
Positive Control	WB: HEK293, A549, Jurkat, A431, HeLa,NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, High Performance, Recombinant, Validated
Product Description	Rabbit anti Human MEK1/MEK2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/5000-1/10000 IHC: 1/100-1/500 ICC/IF: 1/50-1/100 Protein Function： Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MAP2K2 Tclin Dual specificity mitogen-activated protein kinase kinase 2 (0 Activities)

Discover Target: MAP2K2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAP2K1 Tclin Dual specificity mitogen-activated protein kinase kinase 1 (0 Activities)

Discover Target: MAP2K1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAP2K2 Tclin Dual specificity mitogen-activated protein kinase kinase 2 (1262 Activities)

Discover Target: MAP2K2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K1 Tclin Dual specificity mitogen-activated protein kinase kinase 1 (4127 Activities)

Discover Target: MAP2K1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K2 Tclin Dual specificity mitogen-activated protein kinase kinase; MEK1/2 (426 Activities)

Discover Target: MAP2K2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K2 Tclin Protein cereblon/MAP2K2 (15 Activities)

Discover Target: MAP2K2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K1 Tclin Protein cereblon/MAP2K1 (15 Activities)

Discover Target: MAP2K1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K2 Tclin VHL/MAP2K2 (53 Activities)

Discover Target: MAP2K2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K1 Tclin VHL/MAP2K1 (57 Activities)

Discover Target: MAP2K1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K2 Tclin VHL-MAP2K1/MAP2K2 (64 Activities)

Discover Target: MAP2K2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - Western Blot
All lanes: Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 42 kDa
Exposure time: 1.1 s

Western Blot: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - Western Blot
All lanes use Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/5000 dilution for 1 hour at room temperature.

IF/ICC: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/50 dilution.

IF/ICC: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/150 dilution.

IF/ICC: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/200 dilution.

IF/ICC: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851).

IHC: Recombinant MEK1/MEK2 Antibody (Ab114851)

Recombinant MEK1/MEK2 Antibody (Ab114851) - IHC
Immunohistochemical analysis of paraffin-embedded human kidney, using Recombinant MEK1/MEK2 Antibody (Ab114851).

Application

Application	Dilution info
IHC	1/100-1/500
WB	1/5000 – 1/10000
IF/ICC	1/50-1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

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Dilution Calculator

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Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

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Recombinant MEK1/MEK2 Antibody - Primary antibody, specific to MEK1/MEK2, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab114851-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab114851-50μl	50μl	1	$199.90
Ab114851-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$319.90
Ab114851-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,099.90

Recombinant MEK1/MEK2 Antibody - Primary antibody, specific to MEK1/MEK2, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews