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Ab091154-10μl

10μl

49,90$US

Ab091154-50μl

50μl

147,90$US

Ab091154-100μl

100μl

237,90$US

Ab091154-1ml

1ml

Available within 8-12 weeks(?)

1 707,90$US

Description générale

Product Name	Recombinant beta III Tubulin Antibody - Primary antibody, specific to TUBB3, Rabbit IgG
Synonymes	beta 3 tubulin antibody \| beta 4 antibody \| beta-4 antibody \| CDCBM antibody \| CDCBM1 antibody \| CFEOM3 antibody \| CFEOM3A antibody \| FEOM3 antibody \| M(beta)3 antibody \| M(beta)6 antibody \| MC1R antibody \| Neuron specific beta III Tubulin antibody \| Neur
Spécifications et pureté	ExactAb™, Validated, Recombinant, High performance, 0.088 mg/mL
Espèces hôtes	Rabbit
Spécificité	TUBB3
Immunogène	A synthetic peptide corresponding to residues on the C-terminus of human beta III tubulin was used as an immunogen.
Contrôle positif	WB: Jurkat, HeLa, NIH/3T3, C6, HEK293, HUVEC and A549 cell lysates. Flow: HeLa cells.
Conjugaison	Unconjugated
Grade	ExactAb™, High Performance, Recombinant, Validated
Product Description	Rabbit anti Human Beta III Tubulin Antibody, Recombinant (RR607), could be used for WB, Flow and so on. Application: WB: 1/2000 - 1/5000 Flow: 1/200 - 1/1000 Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Post-translational Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

Propriétés du produit

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Méthode de purification	Protein A purified
Forme	Liquid
Concentration	0.088 mg/mL
Température de stockage	Store at -20°C,Avoid repeated freezing and thawing
Expédié en	Ice chest + Ice pads
Stabilité et stockage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Cibles associées (humaines)

TUBB3 Tclin Tubulin beta-3 chain (0 Activities)

Discover Target: TUBB3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	Références

Images

Western Blot: Recombinant beta III Tubulin Antibody (Ab091154)

Recombinant beta III Tubulin Antibody (Ab091154) - Western Blot
All lanes: Recombinant beta III Tubulin Antibody (Ab091154) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 6.3 s

Flow Cytometry: Recombinant beta III Tubulin Antibody (Ab091154)

Recombinant beta III Tubulin Antibody (Ab091154) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant beta III Tubulin Antibody (Ab091154) (gold). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant beta III Tubulin Antibody (Ab091154) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Red) was used as a control.

Application

Application	Dilution info
Flow Cytometry	1/200 - 1/1000
WB	1/2000 - 1/5000

Certificats (CoA, COO, BSE/TSE et tableau d'analyse)

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2 results found

Lot Number	Certificate Type	Date	Article
ZJ23F0901098	Certificate of Analysis	Sep 18, 2023	Ab091154
ZJ23F0901099	Certificate of Analysis	Sep 18, 2023	Ab091154

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Flow cytometry protocol

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Bielschowsky Silver Staining Technique

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Recombinant beta III Tubulin Antibody - Primary antibody, specific to TUBB3, Rabbit IgG

Description générale

Propriétés du produit

Cibles associées (humaines)

Mécanismes d'action

Images

Application

Certificats (CoA, COO, BSE/TSE et tableau d'analyse)

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Recombinant beta III Tubulin Antibody - Primary antibody, specific to TUBB3, Rabbit IgG

Description générale

Propriétés du produit

Cibles associées (humaines)

Mécanismes d'action

Images

Application

Certificats (CoA, COO, BSE/TSE et tableau d'analyse)

Produits apparentés

Nous avons trouvé d’autres produits qui pourraient vous intéresser !

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Calculateurs de solution

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