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Goat Anti-Mouse IgG H&L (PE) - Secondary antibody, Goat IgG

1 Citations COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Mouse
  • Isotype: Goat IgG
  • Conjugation: PE
In stock
Item Number
Ab179006
Grouped product items
SKU Size
Availability
 Price Qty
Ab179006-100μl
100μl
≥10
$59.90
Ab179006-1ml
1ml
1
$259.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Mouse IgG H&L (PE); IF, Flow

View related series
CLARITY tissue clearing technology (64) Flow Cytometry (1158) PE (167)

Basic Description

Product Name Goat Anti-Mouse IgG H&L (PE) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Mouse IgG H&L Antibody (PE) | Anti-Mouse IgG H&L Antibody (PE) | Mouse IgG H&L Antibody (PE) | PE-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (PE) | Anti-Mouse IgG Antibody (PE) | Mouse IgG Antibody (PE) | Goat anti-Mouse
Specifications & Purity ExactAb™, High Performance, Validated, Azide Free, Ex:565nm, Em:575nm, 1.0 mg/mL
Host species Goat
Immunogen Mouse IgG
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade Azide Free, Em:575nm, Ex:565nm, ExactAb™, High Performance, Validated
Product Description

Application
IF: 1:1000
Flow: 1:100-1:2500

Product Properties

Isotype Goat IgG
Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In Wet ice
Stability And Storage Store at 4℃ for one year. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Mouse IgG H&L (PE) (Ab179006) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (PE) (Ab179006) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (PE) (Ab179006) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
IF/ICC

1:1000
Flow Cytometry

1:100-1:2500

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0102529 Certificate of Analysis Jan 17, 2024 Ab179006
ZJ24F0102530 Certificate of Analysis Jan 17, 2024 Ab179006
ZJ24R0100214 Certificate of Analysis Jan 13, 2024 Ab179006

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Documents & Articles

Immunocytochemistry (ICC) protocol
Categories: Technical articles
Tags:
Antibody Biological buffer Fluorescent dyes Immunochemical staining Indirect antibody labeling
RNA dot blot protocol
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies
Caspase Immunofluorescence Staining Protocol
Categories: Protocols
Tags:
Secondary antibody Apoptosis Programmed Death Immunofluorescence Caspase
General Protocol for Western Blotting
Categories: Protocols
Tags:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP
Western Blotting with Fluorescently Labeled Antibodies
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
Immunofluorescence Staining Protocol (Methanol permeabilization)
Categories: Protocols
Tags:
Frozen section Immunofluorescence staining Tissue section Methanol permeability IF
Guidelines of Secondary Antibody for Western Blotting
Categories: Protocols
Tags:
Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Flow Cytometry Using Methanol Permeabilization
Categories: Protocols
Tags:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
CLARITY Tissue Clearing Staining Technology
Categories: Protocols
Tags:
CLARITY tissue clearing technology CLARITY tissue clearing lipid removal biological tissue 3D imaging Hydrogel
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Categories: Protocols
Tags:
Flow Cytometry Transcription Factors Transcription factor flow cytometry Cell fixation and permeabilization Cell fixation Cell permeabilization FOXP3

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936). 

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