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Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG

    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Mouse
  • Isotype: Goat IgG
  • Conjugation: AF555
In stock
Item Number
Ab181950
Grouped product items
SKU Size
Availability
Price Qty
Ab181950-10μg
10μg
5
$15.90
Ab181950-100μg
100μg
5
$59.90
Ab181950-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$349.90

pAb; Goat Anti-Mouse IgG H&L Antibody (AF555); IF, Flow

Basic Description

Product Name Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG
Synonyms Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I
Specifications & Purity ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL
Host species Goat
Specificity Mouse IgG
Immunogen Mouse IgG
Conjugation AF555
Excitation(nm) Green Laser(532nm)
Emission(nm) 568nm
Ex/Em(nm) Ex:553nm;Em:568nm
Grade Em:568nm, Ex:553nm, ExactAb™, Validated
Product Description

Goat Anti-Mouse IgG H&L Antibody, Polyclonal / Secondary antibody, could be uesd for Flow, IF.

Product Properties

Isotype Goat IgG
Purification Method Antigen affinity chromatography
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Mouse IgG H&L (AF555) (Ab181950) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 0.5 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF555) (Ab181950) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (AF555) (Ab181950) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (AF555) (Ab181950) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

1:100-1:2000

IF/ICC

1/100-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Alternative Products

Citations of This Product

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936). 

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