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Goat Anti-Human IgG (FITC) - Secondary antibody

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Human
  • Conjugation: FITC
In stock
Item Number
Ab175834
Grouped product items
SKU Size
Availability
 Price Qty
Ab175834-100μg
100μg
5
$19.90
Ab175834-1mg
1mg
2
$99.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Human IgG (FITC); IF, Flow

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Basic Description

Product Name Goat Anti-Human IgG (FITC) - Secondary antibody
Synonyms Goat Anti-Human Antibody (FITC) | FITC-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / FITC antibody | Goat Anti-Human IgG-FITC antibody
Specifications & Purity ExactAb™, Validated, Azide Free, Ex:498nm, Em:517nm, 1.0 mg/mL
Host species Goat
Conjugation FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm
Grade Azide Free, Em:517nm, Ex:498nm, ExactAb™, Validated
Product Description

Application
Flow: 1:100-1:1000
IF: 1:1000

Product Properties

Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Human IgG (FITC) (Ab175834) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Rituximab (anti-CD20) (R412001). Goat Anti-Human IgG (FITC) (Ab175834) at a dilution of 1/500 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
IF/ICC

1:1000
Flow Cytometry

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001388 Certificate of Analysis Jul 30, 2024 Ab175834
ZJ23F1001387 Certificate of Analysis Oct 26, 2023 Ab175834

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Documents & Articles

Immunocytochemistry (ICC) protocol
Categories: Technical articles
Tags:
Antibody Biological buffer Fluorescent dyes Immunochemical staining Indirect antibody labeling
RNA dot blot protocol
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Caspase Immunofluorescence Staining Protocol
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General Protocol for Western Blotting
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Western Blotting with Fluorescently Labeled Antibodies
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Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
Guidelines of Secondary Antibody for Western Blotting
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Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Flow Cytometry Using Methanol Permeabilization
Categories: Protocols
Tags:
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CLARITY Tissue Clearing Staining Technology
Categories: Protocols
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Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
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