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Goat Anti-Human IgG (AF555) - Secondary antibody, IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Human
  • Isotype: Goat IgG
  • Conjugation: AF555
In stock
Item Number
Ab181961
Grouped product items
SKU Size
Availability
 Price Qty
Ab181961-10μg
10μg
5
$15.90
Ab181961-100μg
100μg
5
$59.90
Ab181961-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$359.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Goat Anti-Human IgG H&L Antibody (AF555); IF, Flow

View related series
AF555 (60) CLARITY tissue clearing technology (64) Flow Cytometry (1158) Goat Anti-Human IgG H&L (1) Goat Anti-Human IgG H&L (AF555) (1)

Basic Description

Product Name Goat Anti-Human IgG (AF555) - Secondary antibody, IgG
Synonyms Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I
Specifications & Purity ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL
Host species Goat
Conjugation AF555
Excitation(nm) Green Laser(532nm)
Emission(nm) 568nm
Ex/Em(nm) Ex:553nm;Em:568nm
Grade Em:568nm, Ex:553nm, ExactAb™, Validated
Product Description

Goat Anti-Human IgG Antibody (AF555), Secondary antibody, could be used for IF, Flow

Product Properties

Isotype IgG
Purification Method antigen affinity chromatography
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Human IgG (AF555) (Ab181961) - IF
IF analysis of EGFR (red) in A431 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Modotuximab (anti-EGFR) (Ab182765) at 1 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF555) (Ab181961) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Human IgG (AF555) (Ab181961) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with Modotuximab (anti-EGFR) (Ab182765). Goat Anti-Human IgG (AF555) (Ab181961) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

1/200-1/2000
IF/ICC

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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