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CD86 Rat mAb (Biotin) - Primary antibody, specific to CD86, Rat IgG2a

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: GL1
  • Conjugation: Biotin
In stock
Item Number
Ab185163
Grouped product items
SKU Size
Availability
Price Qty
Ab185163-10μg
10μg
2
$19.90
Ab185163-25μg
25μg
3
$39.90
Ab185163-100μg
100μg
1
$99.90

mAb (GL1); Rat anti Mouse CD86 Antibody (Biotin); Flow

Basic Description

Product Name CD86 Rat mAb (Biotin) - Primary antibody, specific to CD86, Rat IgG2a
Synonyms Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Rat
Specificity CD86
Immunogen IL-7Ra-IgG1 fusion protein.
Positive Control Flow: Mouse splenocytes.
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Application:
Flow: ≤0.3 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

Isotype Rat IgG2a
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD86 Rat mAb (Biotin) (Ab185163) - Flow Cytometry
Flow cytometry analysis of CD86 expression by LPS-stimulated (1 day) C57BL/6 murine splenocytes (red) compared to an isotype control (blue). Cells were stained with CD86 Rat mAb (Biotin) (Ab185163) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0809152 Certificate of Analysis Aug 02, 2024 Ab185163
ZJ24F0809151 Certificate of Analysis Aug 02, 2024 Ab185163
ZJ24F0809150 Certificate of Analysis Aug 02, 2024 Ab185163
ZJ24R0700824 Certificate of Analysis Jul 18, 2024 Ab185163

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