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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab215324-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$47.90
|
|
|
Ab215324-25μg
|
25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$95.90
|
|
|
Ab215324-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$216.90
|
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mAb (GL1); Rat anti Mouse CD86 Antibody (AF647); Flow Cytometry
| Product Name | CD86 Rat mAb (AF647) - Primary antibody, specific to CD86 |
|---|---|
| Synonyms | Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d |
| Host species | Rat |
| Specificity | CD86 |
| Immunogen | LPS-activated CBA/Ca mouse splenic B cells |
| Positive Control | Flow: C57BL/6 mouse splenocytes |
| Conjugation | AF647 |
| Excitation(nm) | Red Laser(633nm) |
| Emission(nm) | 668nm |
| Ex/Em(nm) | Ex:650nm;Em:668nm |
| Grade | ExactAb™, Validated |
| Product Description |
Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD86 Rat mAb (AF647) (Ab215324) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF647) (Ab215324) (red) compared to an isotype control (blue). Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
CD86 Rat mAb (AF647) (Ab215324) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF647) (Ab215324) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |
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