Grouped product items

SKU

Size

Availability

Price

Qty

Ab215327-10μg

10μg

Available within 8-12 weeks(?)

$49.90

Ab215327-25μg

25μg

Available within 8-12 weeks(?)

$89.90

Ab215327-100μg

100μg

Available within 8-12 weeks(?)

$267.90

Basic Description

Product Name	CD86 Rat mAb (PE-Cy7) - Primary antibody, specific to CD86
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d
Host species	Rat
Specificity	CD86
Immunogen	LPS-activated CBA/Ca mouse splenic B cells
Positive Control	Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation	PE-Cy7
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	774nm
Ex/Em(nm)	Ex:565nm;Em:774nm
Grade	ExactAb™, Validated
Product Description	Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: CD86 Rat mAb (PE-Cy7) (Ab215327)

CD86 Rat mAb (PE-Cy7) (Ab215327) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD86 Rat mAb (PE-Cy7) (Ab215327) compared to an isotype control (blue). Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (PE-Cy7) (Ab215327)

CD86 Rat mAb (PE-Cy7) (Ab215327) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD86 Rat mAb (PE-Cy7) (Ab215327) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Application

Application	Dilution info
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ25F0421023	Certificate of Analysis	Apr 29, 2025	Ab215327
ZJ25F0421024	Certificate of Analysis	Apr 29, 2025	Ab215327

Alternative Products

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CD86 Rat mAb (PE-Cy7) - Primary antibody, specific to CD86

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab215327-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
Ab215327-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$89.90
Ab215327-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$267.90

CD86 Rat mAb (PE-Cy7) - Primary antibody, specific to CD86

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews