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CD86 Rat mAb (APC-Cy7) - Primary antibody, specific to CD86

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: GL1
  • Conjugation: APC-Cy7
In stock
Item Number
Ab215329
Grouped product items
SKU Size
Availability
Price Qty
Ab215329-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab215329-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$95.90
Ab215329-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$273.90

mAb (GL1); Rat anti Mouse CD86 Antibody (APC-Cy7); Flow Cytometry

Basic Description

Product Name CD86 Rat mAb (APC-Cy7) - Primary antibody, specific to CD86
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d
Host species Rat
Specificity CD86
Immunogen LPS-activated CBA/Ca mouse splenic B cells
Positive Control Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation APC-Cy7
Excitation(nm) Red Laser(633nm)
Emission(nm) 774nm
Ex/Em(nm) Ex:650nm;Em:774nm
Grade ExactAb™, Validated
Product Description

Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.


Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD86 Rat mAb (APC-Cy7) (Ab215329) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD86 Rat mAb (APC-Cy7) (Ab215329) (red) compared to an isotype control (blue). Blue - Rat IgG (APC-Cy7) (Ab213859). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

CD86 Rat mAb (APC-Cy7) (Ab215329) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD86 Rat mAb (APC-Cy7) (Ab215329) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (APC-Cy7) (Ab213859).

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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