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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab183891-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
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Ab183891-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
|
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Ab183891-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab183891-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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mAb (AB01/4F12); Mouse anti Human c-Kit; WB, IF/ICC, ELISA; Unconjugated
| Product Name | c-Kit Mouse mAb - Primary antibody, specific to Human c-Kit |
|---|---|
| Synonyms | C Kit antibody | c-Kit antibody | c-Kit Ligand antibody | CD117 antibody | Kit antibody | Kit Ligand antibody | KIT oncogene antibody | KIT proto oncogene receptor tyrosine kinase antibody | KIT_HUMAN antibody | Mast cell growth factor receptor antibody | |
| Specifications & Purity | ExactAb™, Animal Free, Carrier Free, High Performance, Validated, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration |
| Host species | Mouse |
| Specificity | Human c-Kit |
| Immunogen | Recombinant protein corresponding to Human c-Kit |
| Positive Control | WB: K562, HeLa and Jurkat whole cell lysates IF/ICC: HEL cells |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human c-Kit Antibody, clone AB01/4F12, could be used for WB, IF/ICC, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
c-Kit Mouse mAb (Ab183891) - Western Blot
All lanes: c-Kit Mouse Antibody (Ab183891) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 109 kDa
Observed band size: 97 kDa
Exposure time: 91.5 s
c-Kit Mouse mAb (Ab183891) - ICC/IF
IF analysis of c-Kit (Green) in HEL cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with c-Kit Mouse mAb (Ab183891) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/10000
|
| IF/ICC | 1/200 |
Starting at $59.90
Starting at $239.90
Starting at $69.90
Starting at $59.90