Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab090922-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$76.90
|
|
|
Ab090922-50μl
|
50μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$219.90
|
|
|
Ab090922-100μl
|
100μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$321.90
|
|
|
Ab090922-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,937.90
|
|
mAb (2A4); Mouse anti Human BECN1 Antibody; WB, IHC, Flow Cytometry, ELISA; Unconjugated
| Product Name | BECN1 Mouse mAb - Primary antibody, specific to BECN1, IgG1 |
|---|---|
| Synonyms | Coiled-coil myosin-like BCL2-interacting protein | Protein GT197 | APG6 antibody | ATG 6 antibody | ATG6 antibody | ATG6 autophagy related 6 homolog antibody | Bcl-2-interacting protein beclin antibody | Beclin 1 (coiled coil moesin like BCL2 interacting |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Biochemical and Physiological Mechanisms | Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved |
| Host species | Mouse |
| Specificity | BECN1 |
| Immunogen | Recombinant protein of human BECN1 (AA 51-248). |
| Positive Control | WB: Hela, A431, MCF-7, Raji, Jurkat and SKBR-3 cell lysate; IHC: breast cancer tissues and liver cancer tissues; Flow: Raji cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human BECN1 Antibody, Monoclonal (2A4), could be used for WB, IHC, Flow Cytometry, ELISA and so on.
|
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
BECN1 Mouse mAb (Ab090922) - Western Blot
All lanes: BECN1 Mouse mAb (Ab090922) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
BECN1 Mouse mAb (Ab090922) - Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 Mouse mAb (Ab090922)
and negative control (purple).
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC |
1/50-1/200
|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
| Flow Cytometry |
1/200-1/400
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 01, 2023 | Ab090922 | |
| Certificate of Analysis | Aug 01, 2023 | Ab090922 |
Starting at $69.90