Recombinant Human CD40 Ligand/TNFSF5 Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P29965
蛋白标签: N-His
生物活性: Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp169583) with the EC50 of 25.02 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp189423-10μg	10μg	现货
rp189423-50μg	50μg	现货
rp189423-100μg	100μg	现货
rp189423-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD40LG

Accession#:P29965 (11) CD40 Ligand/TNFSF5 (1) CD40LG (11) Gene ID:959 (11)

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基本描述

产品名称	Recombinant Human CD40 Ligand/TNFSF5 Protein
别名	重组人CD40 Ligand/TNFSF5蛋白 \| 重组人 CD40 配体/TNFSF5 蛋白
英文别名	CD154 \| CD40 antigen ligand \| CD40 Ligand \| CD40L \| CD40-L \| CD40LG \| CD40LIGM \| gp39 \| CD154 antigen \| hCD40L \| HIGM1 \| T-B cell-activating molecule \| T-BAM \| T-cell antigen Gp39 \| TNF-related activation protein \| TNFSF5 \| TNFSF5IMD3 \| TRAP \| TRAPtumor n
规格或纯度	ActiBioPure™, Bioactive, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function: CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
生物活性	Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp169583) with the EC50 of 25.02 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	108-261 aa
序列	MHHHHHHENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P29965
来源	重组表达
预测分子量	17.7 kDa
SDS-PAGE	15.4 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM Tris, 150mM NaCl, pH 8.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Binding Activity: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - Binding Activity
Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp189423) with the EC50 of 25.02 ng/mL.

Binding Activity: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423)

ELISA: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - ELISA
Immobilized Recombinant Human CD40 Ligand/TNFSF5 protein (rp189423) at 1.0 μg/mL can bind Dapirolizumab (anti-CD40L) (Ab191960) with the EC₅₀ of 28.88 ng/mL.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0708542	分析证书	rp189423
ZJ24F0708543	分析证书	rp189423
ZJ24F0708544	分析证书	rp189423

技术文档和文章

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Recombinant Human CD40 Ligand/TNFSF5 Protein

库存信息

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库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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溶液计算器

摩尔浓度计算器

稀释计算器

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