货号 (SKU)

包装规格

是否现货

价格

数量

rp152636-10μg

10μg

现货

rp152636-50μg

50μg

现货

rp152636-100μg

100μg

现货

rp152636-1mg

1mg

期货

基本描述

产品名称	Recombinant Human CD40 Ligand/TNFSF5 Protein
别名	重组人CD40配体蛋白 \| 重组人 CD40 配体/TNFSF5 蛋白
英文别名	CD154 antigen \| CD154 \| CD40 antigen ligand \| CD40 Ligand \| CD40L \| CD40-L \| CD40LG \| CD40LIGM \| gp39 \| hCD40L \| HIGM1 \| T-B cell-activating molecule \| T-BAM \| T-cell antigen Gp39 \| TNF-related activation protein \| TNFSF5 \| TNFSF5IMD3 \| TRAP \| TRAPtumor n
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE&HPLC)
产品介绍	Purity >95% (SDS-PAGE&HPLC) Endotoxin level <1.0 EU/μg Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	113-263 aa
序列	MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
纯度	>95% (SDS-PAGE&HPLC)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	P29965
来源	重组表达
预测分子量	16.3 kDa
SDS-PAGE	16.3 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 1*PBS, pH 7.0.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	自收到之日起 12 个月，-20 至 -70 °C。1 个月，复溶后无菌条件下 2 至 8 °C。3 个月，复溶后在无菌条件下 -20 至 -70 °C。收货后建议分装。避免冻融循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636) - Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/mL.

SDS-PAGE: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636) - SDS-PAGE
Recombinant Human CD40 Ligand/TNFSF5 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 16.3 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001353	分析证书	rp152636
ZJ23F1001351	分析证书	rp152636
ZJ23F1001352	分析证书	rp152636

技术文档和文章

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引用文献

1. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] [10.1021/acs.jmedchem.7b01154]
2. Xiao HY,Li N,Duan JJ,Jiang B,Lu Z,Ngu K,Tino J,Kopcho LM,Lu H,Chen J,Tebben AJ,Sheriff S,Chang CY,Yanchunas J,Calambur D,Gao M,Shuster DJ,Susulic V,Xie JH,Guarino VR,Wu DR,Gregor KR,Goldstine CB,Hynes J,Macor JE,Salter-Cid L,Burke JR,Shaw PJ,Dhar TGM. (2020) Biologic-like In Vivo Efficacy with Small Molecule Inhibitors of TNFα Identified Using Scaffold Hopping and Structure-Based Drug Design Approaches.. J Med Chem, 63 (23): (15050-15071). [PMID:33261314] [10.1021/acs.jmedchem.0c01732]
3. Graf, D D, Korthäuer, U U, Mages, H W HW, Senger, G G and Kroczek, R A RA.. (1992) Cloning of TRAP, a ligand for CD40 on human T cells.. European journal of immunology, [PMID:1280226]
4. Spriggs, M K MK and 7 more authors.. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. The Journal of experimental medicine, (1): [PMID:1281209]
5. Hollenbaugh, D D and 8 more authors.. (1992) The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. The EMBO journal, [PMID:1385114]
6. Saiki, O O and 9 more authors.. (1995) Signaling through CD40 rescues IgE but not IgG or IgA secretion in X-linked immunodeficiency with hyper-IgM.. The Journal of clinical investigation, [PMID:7532185]
7. Gauchat, J F JF and 6 more authors.. (1993) Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.. FEBS letters, (11): [PMID:7678552]
8. Aruffo, A A and 9 more authors.. (1993) The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell, (29): [PMID:7678782]
9. Korthäuer, U U and 9 more authors.. (1993) Defective expression of T-cell CD40 ligand causes X-linked immunodeficiency with hyper-IgM.. Nature, (11): [PMID:7679206]
10. Allen, R C RC and 9 more authors.. (1993) CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome.. Science (New York, N.Y.), (12): [PMID:7679801]
11. Macchi, P P and 9 more authors.. (1995) Characterization of nine novel mutations in the CD40 ligand gene in patients with X-linked hyper IgM syndrome of various ancestry.. American journal of human genetics, [PMID:7717401]
12. Shimadzu, M M and 6 more authors.. (1995) Structural organization of the gene for CD40 ligand: molecular analysis for diagnosis of X-linked hyper-IgM syndrome.. Biochimica et biophysica acta, (2): [PMID:7999797]
13. DiSanto, J P JP, Bonnefoy, J Y JY, Gauchat, J F JF, Fischer, A A and de Saint Basile, G G.. (1993) CD40 ligand mutations in x-linked immunodeficiency with hyper-IgM.. Nature, (11): [PMID:8094231]
14. Lin, Q Q and 9 more authors.. (1996) A single strand conformation polymorphism study of CD40 ligand. Efficient mutation analysis and carrier detection for X-linked hyper IgM syndrome.. The Journal of clinical investigation, (1): [PMID:8550833]
15. Karpusas, M M and 6 more authors.. (1995) 2 A crystal structure of an extracellular fragment of human CD40 ligand.. Structure (London, England : 1993), (15): [PMID:8589998]
16. Blotta, M H MH, Marshall, J D JD, DeKruyff, R H RH and Umetsu, D T DT.. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8617933]
17. Pietravalle, F F and 7 more authors.. (1996) Human native soluble CD40L is a biologically active trimer, processed inside microsomes.. The Journal of biological chemistry, (15): [PMID:8626375]
18. Katz, F F and 5 more authors.. (1996) Mutation analysis in CD40 ligand deficiency leading to X-linked hypogammaglobulinemia with hyper IgM syndrome.. Human mutation, [PMID:8889581]
19. Nonoyama, S S and 7 more authors.. (1997) Mutations of the CD40 ligand gene in 13 Japanese patients with X-linked hyper-IgM syndrome.. Human genetics, [PMID:9150729]
20. Singh, J J and 7 more authors.. (1998) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.. Protein science : a publication of the Protein Society, [PMID:9605317]
21. Seyama, K K and 6 more authors.. (1998) Mutations of the CD40 ligand gene and its effect on CD40 ligand expression in patients with X-linked hyper IgM syndrome.. Blood, (1): [PMID:9746782]
22. Khandekar, S S SS and 9 more authors.. (2001) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.. Protein expression and purification, [PMID:11676606]
23. Mikolajczak, Sebastian A SA and 5 more authors.. (2004) The modulation of CD40 ligand signaling by transmembrane CD28 splice variant in human T cells.. The Journal of experimental medicine, (5): [PMID:15067037]
24. Ouadani, Hanen H and 14 more authors.. (2016) Novel and recurrent AID mutations underlie prevalent autosomal recessive form of HIGM in consanguineous patients.. Immunogenetics, [PMID:26545377]
25. Takada, Yoko K; Yu, Jessica; Shimoda, Michiko and Takada, Yoshikazu.. (2019) Integrin Binding to the Trimeric Interface of CD40L Plays a Critical Role in CD40/CD40L Signaling.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:31331973]

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所需的复溶浓度

Recombinant Human CD40 Ligand/TNFSF5 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

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