货号 (SKU)

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数量

rp169583-10μg

10μg

现货

rp169583-50μg

50μg

现货

rp169583-1mg

1mg

现货

rp169583-100μg

100μg

现货

基本描述

产品名称	Recombinant Human CD40 Ligand/TNFSF5 Protein
别名	重组人CD40 Ligand/TNFSF5蛋白 \| 重组人 CD40 配体/TNFSF5 蛋白
英文别名	CD154 antigen \| CD154 \| CD40 antigen ligand \| CD40 Ligand \| CD40L \| CD40-L \| CD40LG \| CD40LIGM \| gp39 \| hCD40L \| HIGM1 \| T-B cell-activating molecule \| T-BAM \| T-cell antigen Gp39 \| TNF-related activation protein \| TNFSF5 \| TNFSF5IMD3 \| TRAP \| TRAPtumor n
规格或纯度	ActiBioPure™, Bioactive, Carrier Free, Azide Free, ≥98%(SDS-PAGE)
产品介绍	Purity:>98%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
生物活性	Testing in progress
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	108-261 aa
序列	MHHHHHHENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
纯度	>98% SDS-PAGE
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P29965
来源	重组表达
预测分子量	17.7 kDa
SDS-PAGE	16.0 kDa, reducing conditions; 16.0 kDa, non-reducing conditions

储存与运输

物理形态	透明胶状
储存缓冲液	Lyophilized from 10mM PB, 500mM NaCl,10% Glycerol
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp169583)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp169583) - SDS-PAGE
Recombinant Human CD40 Ligand/TNFSF5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.0 kDa.

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找到5个结果

批号(Lot Number)	证书类型	货号
ZJ23F1201884	分析证书	rp169583
ZJ23F1201882	分析证书	rp169583
ZJ23F1201883	分析证书	rp169583
ZJ23F1201885	分析证书	rp169583
ZJ23F1201881	分析证书	rp169583

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引用文献

1. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] [10.1021/acs.jmedchem.7b01154]
2. Xiao HY,Li N,Duan JJ,Jiang B,Lu Z,Ngu K,Tino J,Kopcho LM,Lu H,Chen J,Tebben AJ,Sheriff S,Chang CY,Yanchunas J,Calambur D,Gao M,Shuster DJ,Susulic V,Xie JH,Guarino VR,Wu DR,Gregor KR,Goldstine CB,Hynes J,Macor JE,Salter-Cid L,Burke JR,Shaw PJ,Dhar TGM. (2020) Biologic-like In Vivo Efficacy with Small Molecule Inhibitors of TNFα Identified Using Scaffold Hopping and Structure-Based Drug Design Approaches.. J Med Chem, 63 (23): (15050-15071). [PMID:33261314] [10.1021/acs.jmedchem.0c01732]
3. Graf, D D, Korthäuer, U U, Mages, H W HW, Senger, G G and Kroczek, R A RA.. (1992) Cloning of TRAP, a ligand for CD40 on human T cells.. European journal of immunology, [PMID:1280226]
4. Spriggs, M K MK and 7 more authors.. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. The Journal of experimental medicine, (1): [PMID:1281209]
5. Hollenbaugh, D D and 8 more authors.. (1992) The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. The EMBO journal, [PMID:1385114]
6. Saiki, O O and 9 more authors.. (1995) Signaling through CD40 rescues IgE but not IgG or IgA secretion in X-linked immunodeficiency with hyper-IgM.. The Journal of clinical investigation, [PMID:7532185]
7. Gauchat, J F JF and 6 more authors.. (1993) Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.. FEBS letters, (11): [PMID:7678552]
8. Aruffo, A A and 9 more authors.. (1993) The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell, (29): [PMID:7678782]
9. Korthäuer, U U and 9 more authors.. (1993) Defective expression of T-cell CD40 ligand causes X-linked immunodeficiency with hyper-IgM.. Nature, (11): [PMID:7679206]
10. Allen, R C RC and 9 more authors.. (1993) CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome.. Science (New York, N.Y.), (12): [PMID:7679801]
11. Macchi, P P and 9 more authors.. (1995) Characterization of nine novel mutations in the CD40 ligand gene in patients with X-linked hyper IgM syndrome of various ancestry.. American journal of human genetics, [PMID:7717401]
12. Shimadzu, M M and 6 more authors.. (1995) Structural organization of the gene for CD40 ligand: molecular analysis for diagnosis of X-linked hyper-IgM syndrome.. Biochimica et biophysica acta, (2): [PMID:7999797]
13. DiSanto, J P JP, Bonnefoy, J Y JY, Gauchat, J F JF, Fischer, A A and de Saint Basile, G G.. (1993) CD40 ligand mutations in x-linked immunodeficiency with hyper-IgM.. Nature, (11): [PMID:8094231]
14. Lin, Q Q and 9 more authors.. (1996) A single strand conformation polymorphism study of CD40 ligand. Efficient mutation analysis and carrier detection for X-linked hyper IgM syndrome.. The Journal of clinical investigation, (1): [PMID:8550833]
15. Karpusas, M M and 6 more authors.. (1995) 2 A crystal structure of an extracellular fragment of human CD40 ligand.. Structure (London, England : 1993), (15): [PMID:8589998]
16. Blotta, M H MH, Marshall, J D JD, DeKruyff, R H RH and Umetsu, D T DT.. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8617933]
17. Pietravalle, F F and 7 more authors.. (1996) Human native soluble CD40L is a biologically active trimer, processed inside microsomes.. The Journal of biological chemistry, (15): [PMID:8626375]
18. Katz, F F and 5 more authors.. (1996) Mutation analysis in CD40 ligand deficiency leading to X-linked hypogammaglobulinemia with hyper IgM syndrome.. Human mutation, [PMID:8889581]
19. Nonoyama, S S and 7 more authors.. (1997) Mutations of the CD40 ligand gene in 13 Japanese patients with X-linked hyper-IgM syndrome.. Human genetics, [PMID:9150729]
20. Singh, J J and 7 more authors.. (1998) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.. Protein science : a publication of the Protein Society, [PMID:9605317]
21. Seyama, K K and 6 more authors.. (1998) Mutations of the CD40 ligand gene and its effect on CD40 ligand expression in patients with X-linked hyper IgM syndrome.. Blood, (1): [PMID:9746782]
22. Khandekar, S S SS and 9 more authors.. (2001) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.. Protein expression and purification, [PMID:11676606]
23. Mikolajczak, Sebastian A SA and 5 more authors.. (2004) The modulation of CD40 ligand signaling by transmembrane CD28 splice variant in human T cells.. The Journal of experimental medicine, (5): [PMID:15067037]
24. Ouadani, Hanen H and 14 more authors.. (2016) Novel and recurrent AID mutations underlie prevalent autosomal recessive form of HIGM in consanguineous patients.. Immunogenetics, [PMID:26545377]
25. Takada, Yoko K; Yu, Jessica; Shimoda, Michiko and Takada, Yoshikazu.. (2019) Integrin Binding to the Trimeric Interface of CD40L Plays a Critical Role in CD40/CD40L Signaling.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:31331973]

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体积（添加到小瓶中）

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Recombinant Human CD40 Ligand/TNFSF5 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

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