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Recombinant Human FGF basic/FGF2/bFGF Protein

  • 表达系统: 水稻(Oryza sativa)
  • Accession #: P09038
  • 蛋白标签: No tag
  • 生物活性: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
  • 内毒素水平: <1.0 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp155946-10μg
10μg 现货 Stock Image
rp155946-50μg
50μg 现货 Stock Image
rp155946-100μg
100μg 现货 Stock Image
rp155946-1mg
1mg 现货 Stock Image

基本描述

产品名称 Recombinant Human FGF basic/FGF2/bFGF Protein
别名 重组人FGF basic/FGF2/bFGF蛋白 | 重组人碱性成纤维细胞生长因子蛋白 | 植物源重组人碱性成纤维细胞生长因子
英文别名 basic fibroblast growth factor bFGF | Basic fibroblast growth factor | bFGF | FGF basic | FGF2 | FGF-2 | FGFBprostatropin | fibroblast growth factor 2 (basic) | HBGF-2 | heparin-binding growth factor 2 | Prostatropin
规格或纯度 无载体, GMP, 生物活性, ActiBioPure™, 高性能, 无动物源, ≥95%(SDS-PAGE)
生化机理 Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. Post-translational: Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
产品介绍

碱性成纤维细胞生长因子(bFGF,FGF2或FGF-β)是成纤维细胞生长因子家族中的一员。bFGF是一种单链多肽生长因子,在促进细胞增殖过程中起着重要作用;同时也可以抑制细胞凋亡,加速伤口愈合并诱导血管再生。在中胚层和神经外胚层等多种类型的细胞中,bFGF亦能非常有效地诱导DNA的合成。OsrhbFGF是运用基因重组技术,由水稻胚乳细胞表达并通过蛋白提纯工艺而成。

Basic fibroblast growth factor (bFGF, FGF2 or FGF-β) is a member of the fibroblast growth factor family. bFGF is a kind of single-chain polypeptide growth factor, which plays an important role in promoting cell proliferation. It can also inhibit cell apoptosis, accelerate wound healing and induce blood vessel regeneration. In many cell types, such as mesoderm and neuroectoderm, bFGF is also very effective in inducing DNA synthesis. OsrhbFGF is expressed by rice endosperm cells and purified by gene recombination technology.

生物活性 Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
内毒素水平 <1.0 EU/μg
表达系统 水稻(Oryza sativa)
种属 人(Human)
氨基酸 134-288 aa
序列 MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
纯度 ≥95% (SDS-PAGE)
蛋白标签 No tag
蛋白长度 Full length protein
无载体 Yes
Accession # P09038
来源 重组表达
预测分子量 17 kDa

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Human basic fibroblast growth factor, L-glutathione, Disodium edesylate, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Sodium chloride, Mannitol, H2O
复溶 It is recommended to redissolve the lyophilized powder in sterile water to 100-200 μg/ml, and further dilute it in other solvents.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20 ℃ for 36 months. Upon delivery aliquot. Avoid freeze/thaw cycle. Upon reconstitution, the product should be stored at 2-8 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
CAS编号和信息 62031-54-3
分子类型 蛋白质

图片

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946) - Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically less than 1.0 ng/mL.

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946)-SDS-PAGE
3μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.0 kDa.

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批号(Lot Number) 证书类型 货号
ZJ25F0521857 分析证书 rp155946
ZJ25F0521856 分析证书 rp155946
ZJ25F0521855 分析证书 rp155946
ZJ25F0420239 分析证书 rp155946
ZJ25F0420238 分析证书 rp155946
ZJ25F0320002 分析证书 rp155946
ZJ25F0218516 分析证书 rp155946
ZJ25F0218515 分析证书 rp155946
ZJ25F0218514 分析证书 rp155946
ZJ23F1101719 分析证书 rp155946
ZJ23F1101720 分析证书 rp155946
ZJ23F1101721 分析证书 rp155946
ZJ23F0800885 分析证书 rp155946
ZJ23F0800884 分析证书 rp155946
ZJ23F0300084 分析证书 rp155946
ZJ23F0800883 分析证书 rp155946
ZJ23F0300083 分析证书 rp155946

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