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Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein

COA COA
  • 表达系统: E. coli
  • Accession #: P09038
  • 蛋白标签: No tag
  • 生物活性: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
  • 内毒素水平: <0.2 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp145939-50μg
 50μg 现货 Stock Image
rp145939-100μg
 100μg 现货 Stock Image
rp145939-1mg
 1mg 现货 Stock Image
rp145939-500µg
 500μg 现货 Stock Image
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点击查看蛋白信息: FGF2
Accession#:P09038 (10) bFGF (5) FGF basic (7) FGF-2 (1) Fgf2 (10) Gene ID:2247 (10)
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基本描述

产品名称 Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein
别名 重组人FGF basic/FGF2/bFGF蛋白 | 重组人碱性成纤维细胞生长因子蛋白 | 植物源重组人碱性成纤维细胞生长因子 | 肝素结合生长因子 | 碱性FGF
英文别名 Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth factor 2 Fibroblast growth factor 2 (basic) Fibroblast growth factor, basic HBGF 2 HBGF-2 HBGF2 HBGH 2 HBGH2 Hepari
规格或纯度 GMP, 生物活性, ActiBioPure™, 无动物源, 无载体, 高性能, ≥95%(SDS-PAGE)
产品介绍

重组人碱性成纤维细胞生长因子(又称为碱性FGF、bFGF、FGF2、FGF-β或者肝素结合生长因子)，是一种适用于细胞培养应用的生物活性蛋白质。bFGF 可调节细胞增殖、分化、存活、粘附、运动、凋亡、肢体形成和伤口恢复等多种过程。bFGF可用于血管发生、成纤维细胞有丝分裂、PC-12 细胞的轴突生长、受体结合以及酪氨酸磷酸化研究。本品系重组大肠杆菌表达、经多步分离纯化，溶解于 10mM PBS, 0.15 M NaCl(pH7.2)溶液中，再经 0.22um 滤膜过滤除菌后冷冻干燥而制成的冻干粉。
应用
配制无血清培养基，
用于细胞培养，
创伤修复药品的生产。
优势
安全性高，
无动物源性：无动物病毒、无致病物质、无外源因子污染，
符合中国药典 2020 版标准。
本品为含生物活性保护剂的冻干粉，
合规性：生产设备和生产环境符合相关法规要求，符合 GMP 指导原则。

Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-beta, or heparin-binding growth factor), is a biologically active protein suitable for cell culture applications. bFGF regulates diverse processes such as cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound recovery. bFGF can be used in studies of angiogenesis, fibroblast mitosis, axonal outgrowth in PC-12 cells, receptor binding, and tyrosine phosphorylation. This strain is expressed in recombinant Escherichia coli, and after multi-step separation and purification, it is dissolved in 10mM PBS, 0.15 M NaCl (pH7.2) solution, filtered through a 0.22 μm filter membrane, and then freeze-dried to make a lyophilized powder.

生物活性 Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
内毒素水平 <0.2 EU/μg
表达系统 E. coli
种属 人(Human)
氨基酸 134-288 aa
序列 MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
纯度 ＞95% SDS-PAGE
蛋白标签 No tag
蛋白长度 Full length protein
无载体 Yes
Accession # P09038
来源 重组表达
预测分子量 17 kDa

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from 10mM PBS, 0.15 M NaCl, pH7.2.
复溶 建议开盖前短暂离心使粉末沉于管底，然后用灭菌过的去离子水溶解，配制为 100μg/ml 的储存液，2℃ ~8℃可稳定保存一周。稀释到较低浓度(不低于 10μg/ml)时，需要加入 0.1%的组织培养级 BSA 或 HSA。 若需延长储存时间，建议在-80℃或-20℃冷冻，不建议重复冷冻和解冻。
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 短期储存2-8℃（4周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
CAS编号和信息 62031-54-3
分子类型 蛋白质

图片

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-SDS-PAGE
2μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 15.6 kDa.

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.

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批号(Lot Number) 证书类型 货号
ZJ25F0521570 分析证书 rp145939
ZJ25F0521457 分析证书 rp145939
ZJ25F0521456 分析证书 rp145939
ZJ23F0400107 分析证书 rp145939
ZJ23F0400108 分析证书 rp145939
ZJ23F0400109 分析证书 rp145939
ZJ23F0400097 分析证书 rp145939

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阿拉丁®靶点蛋白
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细胞培养通用指南
Categories: 实验方案（Protocols）
探索主题:
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成纤维细胞生长因子家族（FGF）
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Trypsin cell freezing DMSO Mammalian Cell line Cell thawing Dimethyl sulfoxide Cryopreservation tube
Mammalian Cell Cryopreservation Protocol
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Trypsin cell freezing DMSO Mammalian Cell line Cell thawing Dimethyl sulfoxide Cryopreservation tube

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引用文献

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