Recombinant Human FGF basic Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P09038
蛋白标签: N-His
生物活性: Measured in a cell proliferation assay using NIH‑3T3 mouse fibroblast cells. The ED50 for this effect is 0.44 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp156010-10μg	10μg	现货
rp156010-50μg	50μg	现货
rp156010-100μg	100μg	现货
rp156010-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: FGF2

Accession#:P09038 (10) FGF basic (7) Fgf2 (10) Gene ID:2247 (10)

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基本描述

产品名称	Recombinant Human FGF basic Protein
别名	重组人FGF basic蛋白 \| 重组人FGF碱性蛋白
英文别名	Basic fibroblast growth factor \| Basic fibroblast growth factor bFGF \| BFGF \| FGF 2 \| FGF B \| FGF-2 \| Fgf2 \| FGF2 basic \| FGF2_HUMAN \| FGFB \| Fibroblast growth factor 2 (basic) \| Fibroblast growth factor 2 \| Fibroblast growth factor, basic \| HBGF 2 \| HBGF
规格或纯度	ActiBioPure™, Bioactive, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules. Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site. The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment. Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases. The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117. There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural. Human 146 aa FGF basic is 97% aa identical to mouse FGF basic.
生物活性	Measured in a cell proliferation assay using NIH‑3T3 mouse fibroblast cells. The ED50 for this effect is 0.44 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	134-288 aa
序列	MGSSHHHHHHSSGLVPRGSHMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
纯度	>95% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P09038
来源	重组表达
预测分子量	19.4 kDa
SDS-PAGE	19.0 kDa, under reducing conditions; 19.0 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM HEPES, 150mM Nacl, 8% Trehalose, pH7.5.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Biological activity: Recombinant Human FGF basic Protein (rp156010)

Recombinant Human FGF basic Protein (rp156010) - Biological activity

Measured in a cell proliferation assay using NIH‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.44 ng/mL.

SDS-PAGE: Recombinant Human FGF basic Protein (rp156010)

Recombinant Human FGF basic Protein (rp156010) - SDS-PAGE
3 μg/lane of Recombinant Human FGF basic Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 19.0 kDa.

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批号(Lot Number)	证书类型	货号
ZJ25F0825502	分析证书	rp156010
ZJ25F0825501	分析证书	rp156010
ZJ25F0825500	分析证书	rp156010

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Recombinant Human FGF basic Protein

库存信息

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基本描述

储存与运输

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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