Recombinant Rat TNF-alpha Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P16599
蛋白标签: C-His
生物活性: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC50 for this effect is typically <5 pg/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp155241-10μg	10μg	现货
rp155241-20μg	20μg	期货
rp155241-50μg	50μg	现货
rp155241-100μg	100μg	现货
rp155241-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: TNF

Accession#:P16599 (3) Gene ID:24835 (3) Tnf (22) TNF-alpha (11)

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基本描述

产品名称

Recombinant Rat TNF-alpha Protein

别名

重组大鼠TNF α蛋白 | 重组大鼠 TNF-alpha 蛋白

英文别名

规格或纯度

ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)

产品介绍

Background：

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69%-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha .

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing.

The membrane form, but not the soluble form, is phosphorylated on serine residues.

Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.

O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

生物活性

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC50 for this effect is typically <5 pg/mL.

内毒素水平

<0.1 EU/μg

表达系统

HEK293

种属

大鼠(Rat)

氨基酸

80-235 aa

序列

MLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIALHHHHHH

纯度

＞95% (SDS-PAGE)

蛋白标签

C-His

N-末端序列

Met

蛋白长度

Full length protein

无动物源

Yes

偶联

Unconjugated

Accession #

P16599

来源

重组表达

预测分子量

18 kDa

SDS-PAGE

15 kDa, under reducing conditions; 15 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	PBS, pH 7.4, 5%Trehalose，5%Mannitol.
复溶	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is recommended to reconstitute to a concentration 0f 0.1-1mg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	建议在无菌条件下于 -20℃~ -80℃ 保存 1 年以上。避免反复冻融循环
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Rat TNF- alpha Protein (rp155241)

Recombinant Rat TNF- alpha Protein (rp155241) - Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC₅₀ for this effect is typically <5 pg/mL.

SDS-PAGE: Recombinant Rat TNF- alpha Protein (rp155241)

Recombinant Rat TNF- alpha Protein (rp155241) - SDS-PAGE
3μg/lane of Recombinant Mouse TNF-alpha was resolved with SDS-PAGE under reducing (R) conditions and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 15 kDa.

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找到6个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215541	分析证书	rp155241
ZJ24F1215542	分析证书	rp155241
ZJ24F1215543	分析证书	rp155241
ZJ23F0600479	分析证书	rp155241
ZJ23F0600480	分析证书	rp155241
ZJ23F0600507	分析证书	rp155241

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Recombinant Rat TNF-alpha Protein

库存信息

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基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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溶液计算器

摩尔浓度计算器

稀释计算器

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