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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp183596-10μg |
10μg |
现货 ![]() |
| |
| rp183596-50μg |
50μg |
现货 ![]() |
| |
| rp183596-100μg |
100μg |
现货 ![]() |
| |
| rp183596-1mg |
1mg |
期货 ![]() |
|
| 产品名称 | Recombinant Rat TNF-alpha Protein |
|---|---|
| 别名 | 重组大鼠TNF-alpha蛋白 | 重组大鼠 TNF-alpha 蛋白 |
| 英文别名 | APC1 | APC1 protein | Cachectin | DIF | Differentiation inducing factor | Macrophage cytotoxic factor | Tnf | TNF superfamily member 2 | TNF superfamily, member 2 | TNF, macrophage derived | TNF, monocyte derived | TNF-a | TNF-alpha | TNFA | TNFA_HUMAN | |
| 规格或纯度 | ActiBioPure™, Carrier Free, 生物活性, High performance, ≥95%(SDS-PAGE) |
| 生化机理 | 与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合的细胞因子。它主要由巨噬细胞分泌,能诱导某些肿瘤细胞系的细胞死亡。在某些条件下,它能刺激细胞增殖并诱导细胞分化(相似)。通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和胰岛素诱导的葡萄糖摄取,诱导脂肪细胞的胰岛素抵抗。诱导脂肪细胞中的 GKAP42 蛋白降解,这是 TNF 诱导的胰岛素抵抗的部分原因(相似)。与 IL1B 和 IL6 协同诱导血管内皮生长因子的产生,从而在血管生成中发挥作用(相似)。促进破骨细胞生成,从而介导骨吸收(相似)。 TNF 细胞内结构域 (ICD) 可诱导树突状细胞产生 IL12。 |
| 生物活性 | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50 for this effect is typically 1.84 pg/mL. |
| 内毒素水平 | <1.0 EU/μg |
| 表达系统 | E. coli |
| 种属 | 大鼠(Rat) |
| 氨基酸 | 80-235 aa |
| 序列 | LRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL |
| 蛋白标签 | No tag |
| 无载体 | Yes |
| 无动物源 | No |
| Accession # | P16599 |
| 来源 | 重组表达 |
| 预测分子量 | 17.2 kDa |
| SDS-PAGE | 14.5 kDa, under reducing conditions; 14.5 kDa, under non-reducing conditions |
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | Lyophilized from PBS, pH 7.4 |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20°C 下可保存 1 年。避免冷冻/解冻循环。 |
| 分子类型 | 蛋白质 |
Recombinant Rat TNF-alpha Protein (rp183596) - Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50
for this effect is typically 1.84 pg/mL.
Recombinant Rat TNF-alpha Protein (rp183596) - SDS-PAGE
3 μg/lane of Recombinant Rat TNF-alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.5 kDa.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp183596 | |
| 分析证书 | rp183596 | |
| 分析证书 | rp183596 |