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Recombinant Human TNF RII/TNFRSF1B Protein

COA COA
  • 表达系统: E. coli
  • Accession #: P20333
  • 蛋白标签: N-His & GST
  • 生物活性: The binding activity of TNFRSF1B and TNFa, the EC50 was 0.0470~0.085 μg/ml.
  • 内毒素水平: <1.0 EU/mg
有货

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货号 (SKU) 包装规格 是否现货 价格 数量
rp152522-5μg
 5μg 现货 Stock Image
rp152522-20μg
 20μg 现货 Stock Image
rp152522-100μg
 100μg 现货 Stock Image
rp152522-1mg
 1mg 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
点击查看蛋白信息: TNFRSF1B
Accession#:P20333 (4) Gene ID:7133 (4) TNF Receptor II (4) TNF RII (3) TNFRSF1B (6)
main product photo

基本描述

产品名称 Recombinant Human TNF RII/TNFRSF1B Protein
别名 肿瘤坏死因子受体超家族成员1B | 重组人 TNF RII/TNFRSF1B 蛋白
英文别名 CD120b antigen | CD120b | Etanercept | p75 TNF receptor | p75TBPII | p75TNFR | soluble TNFR1B variant 1 | TNF RII | TNFBRp80 TNF-alpha receptor | TNF-R2 | TNFR2TNFR1B | TNF-R75 | TNFR80 | TNFRII | TNF-RII | TNF-R-II | TNFR-II | TNFRSF1B | tumor necrosis f
规格或纯度 ActiBioPure™, Bioactive, Carrier Free, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE)
产品介绍


Purity

>95% SDS-PAGE.


Function

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
生物活性 The binding activity of TNFRSF1B and TNFa, the EC50 was 0.0470~0.085 μg/ml.
内毒素水平 <1.0 EU/mg
表达系统 E. coli
种属 人(Human)
氨基酸 298-441 aa
序列 MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGRGSMGYRGSEFAKVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLL
纯度 ＞95%(SDS-PAGE)
蛋白标签 N-His & GST
蛋白长度 Protein fragment
无载体 Yes
Accession # P20333
来源 重组表达
预测分子量 44.8 kDa

储存与运输

应用 Cell culture, Activity Assays, ELISA, SDS-PAGE
物理形态 冻干(Lyophilized)
储存缓冲液 10mM PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
复溶 Reconstitute in ddH2O to a concentration ≤0.1mg/mL. Do not vortex.
储存温度 -80℃储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 避免反复冻融循环。在 2-8℃ 下保存一个月。分装后在 -80℃ 下可保存 12 个月。
分子类型 蛋白质

图片

Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-Protein Bioactivity
Measured by its binding ability in a functional ELISA.Tumor Necrosis Factor Alpha (TNF-α) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNF-α. 

Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-SDS-PAGE
3μg/lane of Recombinant Human VCAM1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 51.3 kDa under reducing conditions and 49 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0600460 分析证书 rp152522
ZJ23F0600462 分析证书 rp152522
ZJ23F0600461 分析证书 rp152522

可替换产品

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引用文献

1. Pennica, D D and 7 more authors..  (1992)  Biochemical properties of the 75-kDa tumor necrosis factor receptor. Characterization of ligand binding, internalization, and receptor phosphorylation..  The Journal of biological chemistry,  (15): [PMID:1328224]
2. Dembic, Z Z and 7 more authors..  (1990)  Two human TNF receptors have similar extracellular, but distinct intracellular, domain sequences..  Cytokine,  [PMID:1966549]
3. Engelmann, H H, Novick, D D and Wallach, D D..  (1990)  Two tumor necrosis factor-binding proteins purified from human urine. Evidence for immunological cross-reactivity with cell surface tumor necrosis factor receptors..  The Journal of biological chemistry,  (25): [PMID:2153136]
4. Smith, C A CA and 8 more authors..  (1990)  A receptor for tumor necrosis factor defines an unusual family of cellular and viral proteins..  Science (New York, N.Y.),  (25): [PMID:2160731]
5. Heller, R A RA and 5 more authors..  (1990)  Complementary DNA cloning of a receptor for tumor necrosis factor and demonstration of a shed form of the receptor..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2166946]
6. Kohno, T T and 8 more authors..  (1990)  A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2172983]
7. Loetscher, H H and 5 more authors..  (1990)  Purification and partial amino acid sequence analysis of two distinct tumor necrosis factor receptors from HL60 cells..  The Journal of biological chemistry,  (25): [PMID:2173696]
8. Suzuki, J J and 5 more authors..  (1994)  Purification of two types of TNF inhibitors in the urine of the patient with chronic glomerulonephritis..  Nephron,  [PMID:8015639]
9. Rothe, M M, Wong, S C SC, Henzel, W J WJ and Goeddel, D V DV..  (1994)  A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor..  Cell,  (26): [PMID:8069916]
10. Beltinger, C P CP and 9 more authors..  (1996)  Physical mapping and genomic structure of the human TNFR2 gene..  Genomics,  (1): [PMID:8661109]
11. Park, Y C YC, Burkitt, V V, Villa, A R AR, Tong, L L and Wu, H H..  (1999)  Structural basis for self-association and receptor recognition of human TRAF2..  Nature,  (8): [PMID:10206649]
12. Tsuchiya, N N, Komata, T T, Matsushita, M M, Ohashi, J J and Tokunaga, K K..  (2000)  New single nucleotide polymorphisms in the coding region of human TNFR2: association with systemic lupus erythematosus..  Genes and immunity,  [PMID:11197692]
13. Morita, C C and 10 more authors..  (2001)  Association of tumor necrosis factor receptor type II polymorphism 196R with Systemic lupus erythematosus in the Japanese: molecular and functional analysis..  Arthritis and rheumatism,  [PMID:11762942]
14. Peral, Belén B, San Millán, José L JL, Castello, Roberto R, Moghetti, Paolo P and Escobar-Morreale, Héctor F HF..  (2002)  Comment: the methionine 196 arginine polymorphism in exon 6 of the TNF receptor 2 gene (TNFRSF1B) is associated with the polycystic ovary syndrome and hyperandrogenism..  The Journal of clinical endocrinology and metabolism,  [PMID:12161545]
15. Pan, Shi S and 5 more authors..  (2002)  Etk/Bmx as a tumor necrosis factor receptor type 2-specific kinase: role in endothelial cell migration and angiogenesis..  Molecular and cellular biology,  [PMID:12370298]
16. Lainez, Begoña B and 6 more authors..  (2004)  Identification and characterization of a novel spliced variant that encodes human soluble tumor necrosis factor receptor 2..  International immunology,  [PMID:14688072]
17. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,  (18): [PMID:16710414]
18. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]

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