Recombinant Human TNF RII/TNFRSF1B Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P20333
蛋白标签: C-hFc
生物活性: Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50 for this effect is typically 25.25 ng/mL in the presence of 1.0 ng/mL Recombinant Human TNF-α (rp156007).
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp179793-10μg	10μg	现货
rp179793-50μg	50μg	现货
rp179793-100μg	100μg	现货
rp179793-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: TNFRSF1B

Accession#:P20333 (4) Gene ID:7133 (4) TNF RII/TNFRSF1B (1) TNFRSF1B (6)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human TNF RII/TNFRSF1B Protein
别名	重组人TNF RII/TNFRSF1B蛋白 \| 重组人 TNF RII/TNFRSF1B 蛋白
英文别名	CD120b \| CD120b antigen \| Etanercept \| p75 TNF receptor \| p75TBPII \| Tumor necrosis factor receptor type II \| tumor necrosis factor receptor superfamily, member 1B \| tumor necrosis factor receptor superfamily member 1B \| p75TNFR \| soluble TNFR1B variant 1
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Protein Function:： Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
生物活性	Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50 for this effect is typically 25.25 ng/mL in the presence of 1.0 ng/mL Recombinant Human TNF-α (rp156007).
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-257 aa
序列	MAPVAVWAALAVGLELWAAAHALPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGDDDDKPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
蛋白标签	C-hFc
无载体	Yes
无动物源	Yes
Accession #	P20333
来源	重组表达
预测分子量	51.5 kDa
SDS-PAGE	60.5-83.3 kDa, under reducing conditions; 119.3-183.3 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human TNF RII/TNFRSF1B Protein (rp179793)

Recombinant Human TNF RII/TNFRSF1B Protein (rp179793) - Protein Bioactivity

Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED₅₀ for this effect is typically 25.25 ng/mL in the presence of 1.0 ng/mL Recombinant Human TNF-α (rp156007).

SDS-PAGE: Recombinant Human TNF RII/TNFRSF1B Protein (rp179793)

Recombinant Human TNF RII/TNFRSF1B Protein (rp179793) - SDS-PAGE
3 μg/lane of Recombinant Human TNF RII/TNFRSF1B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 60.5-83.3 kDa under reducing conditions and 119.3-183.3 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0708865	分析证书	rp179793
ZJ24F0708866	分析证书	rp179793
ZJ24F0708867	分析证书	rp179793

技术文档和文章

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human TNF RII/TNFRSF1B Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器