| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| P598082-100μl |
100μL |
现货  |
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| 产品名称 | PMA（叠氮溴化丙锭）， 20 mM in water |
|---|---|
| 产品介绍 |
PMA是一种高亲和性的DNA结合染料，该染料本身具有微弱的荧光，但与核酸结合后可以发出更为明亮的荧光。它尤其与双链DNA具有高亲和性。PMA不具有细胞膜渗透性，因此可选择性地修饰膜受损的死细胞的 DNA。PMA修饰的DNA经蓝光（~464 nm）光解后，PMA上的光反应性叠氮基转化为高反应性氮烯自由基，与DNA结合位点附近的任何烃部分反应形成稳定的共价氮碳键，从而导致永久性 DNA 修饰（图1）。该修饰过程会使DNA不溶，并使其在随后的基因组DNA提取过程中与细胞碎片一起丢失，进而阻碍死细胞中目标DNA的PCR扩增。残留在溶液中未结合的PMA，在强光照射下与水分子反应分解成无交联活性的羟胺化合物，使羟胺不再能够共价结合DNA，从而不影响PCR扩增。这个特性使得 PMA 可以通过实时定量 PCR（qPCR）的手段，检测多种样本类型包括：细菌、生物膜、酵母、真菌、病毒和真核细胞；与qPCR、NGS、Sanger测序和LAMP技术相结合，广泛应用在食品和水安全和环境测试中。
产品参数： 橙红色液体； Ex (pH 3) = 464 nm (before photolysis)； Ex /Em (after photocrosslinking to nucleic acid) = 510/610 nm 注意事项： 1. 使用前请将产品瞬时离心至管底，再进行后续实验。 2. 试剂盒组分中含有荧光染料，使用及保存过程中注意避光。 3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。 应用范围： 细菌核酸染色 使用方法： 实验材料（自备）：
7. 处理和未处理的活细菌 5000 ×g，离心 10 min，去上清。 示例：
PMA is a DNA binding dye with high affinity. The dye itself has weak fluorescence, but it can emit brighter fluorescence after binding with nucleic acids. It especially has high affinity with double stranded DNA. PMA does not have cell membrane permeability, so it can selectively modify the DNA of dead cells with damaged membranes. After bllight (~464 nm) photolysis of PMA modified DNA, the photoreactive azido group on PMA is converted into highly reactive azene radical, which reacts with any hydrocarbon moiety near the DNA binding site to form a stable covalent nitrogen carbon bond, resulting in permanent DNA modification (Figure 1). This modification process will make DNA insoluble and make it lost together with cell debris in the subsequent genomic DNA extraction process, thus hindering the PCR amplification of target DNA in dead cells. The unbound PMA remaining in the solution reacts with water molecules under strong light irradiation and decomposes into hydroxylamine compounds without cross-linking activity, so that hydroxylamine can no longer covalently bind DNA, thus not affecting PCR amplification. This feature enables PMA to detect a variety of sample types including bacteria, biofilms, yeast, fungi, viruses and eukarYOtic cells by real-time quantitative PCR (qPCR); Combined with qPCR, ngs, Sanger sequencing and lamp technology, it is widely used in food and water safety and environmental testing.
Product parameters：
Product parameters：Orange-red liquid； Ex (pH 3) = 464 nm (before photolysis)； Ex /Em (after photocrosslinking to nucleic acid) = 510/610 nm
Matters needing attention： 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. the components of the kit contain fluorescent dyes. Avoid light during use and storage. 3. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application： Bacterial nucleic acid staining Instruction： Experimental materials ( self-provided )：
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| 储存温度 | 避光,-20°C储存 |
|---|---|
| 运输条件 | 超低温冰袋运输 |
| CAS编号和信息 | P598082 |
| 分子类型 | 未知 |
¥1,279.90
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