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胰蛋白酶 来源于牛胰腺

  • Accession #: P00760
  • 生物活性: Trypsin >450 USP U/mg powder, Chymotrypsin <75 USP U/mg powder
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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
np225024-25g
25g 现货 Stock Image
np225024-100g
100g 现货 Stock Image

基本描述

产品名称 胰蛋白酶 来源于牛胰腺
英文别名 Serine protease 1 | PRSS1
规格或纯度 ActiBioPure™, GMP, 生物活性, EnzymoPure™, 高性能, >450 USP U/mg powder
生化机理 Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
产品介绍

胰蛋白酶是一种丝氨酸蛋白水解酶。胰蛋白酶特异性严密,专一作用于由碱性氨基酸精氨酸及亮氨酸所组成的肽键。易溶于水,不溶于三氯甲烷、乙醇,乙醚和甘油,Ca2+对胰蛋白酶有保护和激活作用。
用途
临床外科用于各种炎症、溃疡、坏疽、创伤、痿孔引起的局部脓肿、水肿、血肿及毒蛇咬伤等症。外科用于治疗癣疥及其他皮肤疾患。内科用于脓胸、肺气肿、支气管炎、支气管喘息等症。一般肌内注射,也可注入胸腔或脓疡痿管空洞内,排脓洗涤,或喷雾吸入用于呼吸道疾患。
除此之外,治疗糖尿病引起的并发症,如肾炎等;治疗骨科疾病。
作为食品加工助剂切割大分子蛋白。
其水解蛋白的特性作为试剂广泛应用于生物、医药、食品等行业的研发、检测领域。

Trypsin is a kind of serine protein hydrolase, exclusive role in composed of alkaline amino acids arginine and leucine peptide bond, soluble in water, insoluble in chloroform, ethanol, ethyl ether, and glycerin, Ca2+ is protection for trypsin and activation function.
Application
As pharmaceutical raw materials manufacturing injection, infusion agent, sprays, clinical surgery used for all sorts of inflammation, ulcers, gangrene, an abscess caused by trauma, impotent, edema, hematoma and snake bites. Surgery for the treatment of tinea scabies and other skin disorders. Internal medicine for pyothorax, emphysema, bronchitis, bronchus wheeze, etc; Treatment of diabetic complications, such as nephritis; Treatment of bone disease.
As a food processing cutting big molecular protein.
As a proteolytic enzyme is widely applied in biology, medicine and food industries research and development, testing field.

生物活性 Trypsin >450 USP U/mg powder, Chymotrypsin <75 USP U/mg powder
种属 牛(Bovine)
Accession # P00760
来源 天然
预测分子量 23.8 kDa

关联靶点(人)

PRSS3 Tchem 胰蛋白酶-3(Trypsin-3) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
PRSS1 Tclin 胰蛋白酶-1(Trypsin-1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
PRSS2 Tchem 胰蛋白酶-2(Trypsin-2) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

储存与运输

物理形态 冻干(Lyophilized)
浓度 >450 USP U/mg powder
储存温度 2-8°C储存,避光,干燥
运输条件 冰袋运输
稳定性与储存 长期储存2-8℃(2年);复溶后建议分装。避光,密封。
CAS编号和信息 9002-07-7
酶学委员会编号 3.4.21.4
单位定义 25℃,pH7.6,反应体系3.0ml (1cm 光路),每分钟酶解BAEE使253nm下的吸收值增加0.003定义为一个USP单位。一个USP单位=3个BTEE单位。
分子类型

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C of A & Other Certificates(BSE/TSE, COO):
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批号(Lot Number) 证书类型 货号
ZJ25F0927261 分析证书 np225024
ZJ25F0927260 分析证书 np225024

技术文档和文章

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