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Recombinant Trypsin
Trypsin is a typical endopeptidase belonging to the serine protease family. Its primary catalytic mechanism involves the specific hydrolysis of peptide bonds at the carboxyl side of basic amino acids (arginine or lysine). In addition, it can cleave amide or ester bonds formed by the carboxyl groups of these amino acids. Owing to its highly efficient protein-degrading activity, trypsin plays an indispensable role in vaccine production and biopharmaceutical manufacturing.
I. Classification
Animal-derived trypsin: Traditionally extracted from bovine or porcine pancreas. While the preparation process is well established, it carries the risk of contamination with exogenous pathogens. Historically, viral particles or viral nucleic acids have been detected in various vaccines.
Recombinant Trypsin: Produced via genetic engineering in animal component–free systems, thereby eliminating pathogen-related risks. It offers superior control over purity and enzymatic activity and has become the global standard in the field.
Figure 1. Schematic representation of trypsin processing in vivo
II. Applications:
Cell culture and passaging: Used for detaching adherent cells while preserving cell integrity and viability, supporting large-scale vaccine production.
Viral protein cleavage: For example, cleaving influenza virus HA protein to maintain viral infectivity and replication efficiency; it serves as a critical cofactor in virus cultivation and vaccine manufacturing.
Recombinant protein production: Selective hydrolysis removes contaminating proteins, improving purity and yield, and enhancing downstream purification efficiency.
Ⅲ.Aladdin Recombinant Trypsin
1. High Safety
Source safety: Produced via recombinant technology, free of animal-derived materials, eliminating the risk of viral contamination (e.g., swine influenza virus, porcine parvovirus).
Process safety: Manufactured using specialized preparation processes, free of endogenous viral contamination and devoid of bacterial, fungal, or mycoplasma contamination.
Storage and transport safety: Lyophilized form ensures stability during transport and storage, minimizing activity loss.
Chemical safety: Contains no protease inhibitors (e.g., PMSF).
2. High Purity
Purification method: High-performance liquid chromatography (HPLC) for superior purity.
Activity specificity: Exhibits only trypsin-specific activity, with no other protease activity.
Name | ID | Grade & Purity |
Recombinant Trypsin | EnzymoPure™, for protein sequencing ≥4500 units/mg | |
Recombinant Porcine Trypsin | Animal Free, Carrier Free, Bioactive, sterile-filtered, ActiBioPure™, Suitable for molecular biology, EnzymoPure™, ≥90%, >10000 U/mg | |
Recombinant Trypsin | EnzymoPure™, ≥3800 units/mg protein | |
Recombinant Trypsin | EnzymoPure™, ≥2500 units/mg | |
Recombinant Trypsin (cell culture grade) | Animal Free, Carrier Free, GMP, PharmPure™, ActiBioPure™, Bioactive, High Performance, EnzymoPure™, for cell culture, ≥95%(SDS-PAGE), ≥3800USP unit/mg pro. | |
Recombinant Trypsin (Pharmacopoeia Grade) | ActiBioPure™, PharmPure™, GMP, Animal Free, Carrier Free, Bioactive, EnzymoPure™, High Performance, ≥95%(SDS-PAGE), ≥3800USP unit/mg pro. | |
Recombinant Heat-stableTrypsin | Animal Free, Carrier Free, GMP, Bioactive, Recombinant, PharmPure™, ActiBioPure™, EnzymoPure™, ≥1000USP units/mg powder; ≥2500USP units/mg protein | |
Rapid-Trypsin (MS) | Animal Free, Carrier Free, EnzymoPure™, Recombinant,suitable for mass spectrometry (MS), ≥95%(SDS-PAGE), ≥3800USP U/mg protein |
Ⅳ.Aladdin Animal-derived Trypsin
Name | ID | Grade & Purity |
Trypsin | EnzymoPure™, 1:250，Tissue Culture Grade | |
Trypsin from porcine pancreas | EnzymoPure™, potency ≥250 units/mg | |
Trypsin from porcine pancreas | ActiBioPure™, Bioactive, High Performance, EnzymoPure™, 1000-2000 U/mg powder | |
Trypsin from porcine pancreas | tablet, 1 mg tablet | |
Trypsin from porcine pancreas | BioReagent, for cell culture, lyophilized powder, 1,000-2,000 BAEE units/mg solid | |
Trypsin from porcine pancreas | ActiBioPure™, Bioactive, High Performance, EnzymoPure™, 13,000-20,000 BAEE U/mg powder | |
Trypsin from porcine pancreas | γ-irradiated, BioReagent, for cell culture, lyophilized powder, 1,000-2,000 BAEE units/mg solid | |
Trypsin solution from porcine pancreas | sterile-filtered, BioReagent, for cell culture, 1 ×, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red | |
Trypsin, Porcine, MS Grade | Proteomics grade ≥10,000 U/mg | |
Trypsin from bovine pancreas | EnzymoPure™, potency ≥2500 units/mg | |
Trypsin from bovine pancreas | Type XI, lyophilized powder,≥6,000 BAEE units/mg protein | |
Trypsin from bovine pancreas | USP, ≥2,500 USP units/mg solid | |
Trypsin from bovine pancreas | ActiBioPure™, Bioactive, GMP, High Performance, EnzymoPure™, >4000 USP U/mg powder | |
Trypsin from bovine pancreas | EnzymoPure™, potency ≥3000 units/mg | |
Trypsin from bovine pancreas | EnzymoPure™, ≥180 units/mg protein | |
Trypsin from bovine pancreas | EnzymoPure™, TPCK Treated, essentially salt-free, lyophilized powder,≥10,000 BAEE units/mg protein | |
Trypsin from bovine pancreas | BioReagent, for cell culture, essentially salt-free, lyophilized powder,≥9,000 BAEE units/mg protein | |
Trypsin from bovine pancreas | ActiBioPure™, GMP, Bioactive, EnzymoPure™, High Performance, >450 USP U/mg powder | |
Trypsin from bovine pancreas(TPCK Treated) | EnzymoPure™, ≥180 units/mg protein (10,350 BAEE/3,450 USP/NF units/mg protein) | |
Trypsin from bovine pancreas(Modified,Sequencing Grade) | EnzymoPure™, ≥150 units/mg protein(at least 8,625 BAEE/2875 USP/NF units/mg protein) | |
Trypsin from bovine pancreas(TPCK-Treated,Irradiated) | EnzymoPure™, ≥180 units/mg protein | |
Trypsin from bovine pancreas(2X,Sterile,Irradiated) | EnzymoPure™, ≥180 units/mg protein (10,350 BAEE/3,450 USP/NF units/mg protein) | |
Trypsin from bovine pancreas(0.22 μm,Filtered) | EnzymoPure™, ≥180 units/mg protein (10,350 BAEE/3,450 USP/NF units/mg protein) | |
Trypsin from bovine pancreas(Purified,Sequencing Grade II) | EnzymoPure™, ≥150 units/mg protein (at least 8,625 BAEE/2875 USP/NF units/mg protein) | |
Trypsin, Human Pancreas | ≥95%(SDS-PAGE), Extinction Coefficient: 1.45 | |
Trypsin from human pancreas | salt-free, lyophilized powder, Each of≥1,000 BAEE units |
Aladdin: https://www.aladdinsci.com/
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