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DNA酶I

25篇引用文献 COA COA
  • Accession #: P00639
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D128586-10KU
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点击查看蛋白信息: DNASE1
DNA Damage (3514) mRNA药物用酶 (26)
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基本描述

产品名称 DNA酶I
别名 脱氧核糖核酸酶 I
规格或纯度 EnzymoPure™, 重组, ≥5000 units/mg protein；来源于牛胰腺
生化机理 DNase I 是一种内切酶，可作用于嘧啶附近的磷酸二酯键，生成末端带有 5′-磷酸的多核苷酸。在 Mg2+ 存在的情况下，DNAse I 可独立裂解 DNA 的每一条链，裂解位点是随机的。在 Mn2+ 存在的情况下，两条 DNA 链会在大致相同的部位被裂解。Mn2+、Ca2+、Co2+ 和 Zn2+ 等二价阳离子是该酶的活化剂。浓度为 5 mM 的 Ca2+ 能使酶稳定，防止蛋白水解。最佳 pH 值为 7 至 8。牛胰腺中的 DNAse I 由 A、B、C 和 D 四种色谱法可区分的成分组成，它们的摩尔比分别为 4:1:1。其中只有少量的 D。已知 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS）6 和肌动蛋白7 可抑制酶的活性。
产品介绍

用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drμgs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Accession # P00639

储存与运输

浓度 ≥5000 units/mg protein；来源于牛胰腺
储存温度 2-8°C储存
运输条件 冰袋运输
CAS编号和信息 9003-98-9

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批号(Lot Number) 证书类型 货号
G1510082 分析证书 D128586

技术文档和文章

CRISPR-Cas9和靶向基因组编辑
Categories: 技术文章
探索主题:
核酸酶 细胞和分子生物学研究 靶向效率 脱靶突变
CRISPR/Cas9 and targeted genome editing
Categories: 技术文章
探索主题:
Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation
CRISPR/Cas9 and targeted genome editing
Categories: 技术文章
探索主题:
Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation
CRISPR/Cas9 and targeted genome editing
Categories: 技术文章
探索主题:
Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation
流式实验样品制备实验指南
Categories: 实验方案（Protocols）
探索主题:
蛋白样品制备 流式细胞术 流式抗体
Experimental Guide of Sample Preparation for Flow Cytometry
Categories: 实验方案（Protocols）
探索主题:
Protein sample preparation Flow Cytometry Antibody FACS
Experimental Guide of Sample Preparation for Flow Cytometry
Categories: 实验方案（Protocols）
探索主题:
Protein sample preparation Flow Cytometry Antibody FACS
CUT&RUN-seq实验方案
Categories: 实验方案（Protocols）
探索主题:
核酸酶 ChIP CUT&RUN ChIC DNA-蛋白质互作 MNase 刀豆球蛋白A ConA磁珠
RNA提取和反转录实验方案
Categories: 实验方案（Protocols）
探索主题:
RNA提取 逆转录 反转录 TRIzol 细胞RNA DNase RNase
RNA Extraction and Reverse Transcription Protocol
Categories: 实验方案（Protocols）
探索主题:
TRIzol DNase RNase RNA extraction Reverse transcription Cellular RNA
RNA Extraction and Reverse Transcription Protocol
Categories: 实验方案（Protocols）
探索主题:
TRIzol DNase RNase RNA extraction Reverse transcription Cellular RNA

此产品的引用文献

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引用文献

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2. Chenfei Han, Yu Xiao, Zhenjiang Liu, Daolin Du, Ming Li.  (2023)  Cascade amplifying aptasensor for positively correlated detecting OTA: Based on DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence.  FOOD CONTROL,  153  (109970).  [10.1016/j.foodcont.2023.109970]
3. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu.  (2023)  Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  241  (124522).  [PMID:37100332] [10.1016/j.ijbiomac.2023.124522]
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6. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu.  (2022)  Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,  111  (2): (170-184).  [PMID:36054309] [10.1002/jbm.a.37440]
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