基本信息

产品名称	Recombinant CD8 alpha Antibody (PE)
别名	白细胞分化抗原8 \| CD8抗体 \| 大鼠抗小鼠CD8 alpha \| CD8 alpha抗体
英文别名	alpha polypeptide (p32) antibody \| CD_antigen=CD8a antibody \| CD8 antibody \| CD8 antigen alpha polypeptide antibody \| CD8 antigen alpha polypeptide (p32) antibody \| CD8 antigen, alpha polypeptide (p32) antibody \| CD8a antibody \| CD8A antigen antibody \| CD
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
宿主种属	大鼠(Rat)
特异性	Mouse CD8α
种属反应性	小鼠(Mouse)
免疫原	Mouse Spleen Cells or Thymocyte Membranes
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	PE
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	575nm
Ex/Em(nm)	Ex:565nm;Em:575nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

CD8A Tbio T 细胞表面糖蛋白 CD8 α 链(T-cell surface glycoprotein CD8 alpha chain) (0 活性数据)

点击查看靶蛋白信息： CD8A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD8 alpha Antibody (PE) (Ab213872)

Recombinant CD8 alpha Antibody (PE) (Ab213872) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD8 alpha Antibody (PE) (Ab213872) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: Recombinant CD8 alpha Antibody (PE) (Ab213872)

Recombinant CD8 alpha Antibody (PE) (Ab213872) - Flow Cytometry
Flow cytometry analysis of CD8 alpha by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD8 alpha Antibody (PE) (Ab213872) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.