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Recombinant CD3 Antibody (AF647)

Recombinant CD3 Antibody (AF647)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2b
克隆号: P49A24
偶联: AF647

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货号 (SKU)	包装规格	是否现货
Ab213111-10μg	10μg	现货
Ab213111-25μg	25μg	现货
Ab213111-100μg	100μg	现货

查看相关系列

点击查看靶蛋白信息: CD3G

Accession#:P11942 (12) AF647 (149) Ctg-3 (10) Ctg3 (10) Gene ID:12502 (12) T3g (10) 流式细胞术 (1163)

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基本信息

产品名称	Recombinant CD3 Antibody (AF647)
别名	CD3单克隆抗体 \| Anti-CD3抗体 \| CD3抗体 \| CD3单抗
英文别名	CD_antigen: CD3e \| CD3 antigen, delta subunit \| CD3 \| CD3d antigen \| CD3d antigen, delta polypeptide (TiT3 complex) \| CD3d molecule, delta (CD3-TCR complex) \| CD3-DELTA \| CD3e antigen \| CD3e antigen, epsilon polypeptide (TiT3 complex) \| CD3e molecule, eps
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).
宿主种属	大鼠(Rat)
特异性	CD3
种属反应性	小鼠(Mouse)
免疫原	γδTCR-positive T-T hybridoma D1
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

CD3G Tclin T 细胞表面糖蛋白 CD3 γ 链(T-cell surface glycoprotein CD3 gamma chain) (0 活性数据)

点击查看靶蛋白信息： CD3G

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD3 Antibody (AF647) (Ab213111)

Recombinant CD3 Antibody (AF647) (Ab213111) - Flow Cytometry
Two-color flow cytometric analysis of CD3 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF647) (Ab190071; Left panel) or Recombinant CD3 Antibody (AF647) (Ab213111; Right panel) at 0.3μg/test.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0218063	分析证书	Ab213111
ZJ25F0218062	分析证书	Ab213111
ZJ25F0218061	分析证书	Ab213111

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