E-Cadherin Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
1.0 mg/mL

应用:

IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: AB03/2B8
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab186487-10μl	10μl	现货
Ab186487-50μl	50μL	现货
Ab186487-100μl	100μL	现货
Ab186487-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: CDH1

Accession#:P12830 (9) Cdh1 (14) E-Cadherin (5) Gene ID:999 (9)

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基本信息

产品名称	E-Cadherin Mouse mAb
别名	钙粘蛋白 1 \| 上皮细胞钙粘素 \| E-钙粘素 \| E-钙粘蛋白 \| 钙粘蛋白 1小鼠单克隆抗体 \| E-钙粘蛋白抗体 \| Anti-CDH1抗体 \| E-Cadherin抗体 \| 钙粘蛋白 1 \| 上皮细胞钙粘素 \| E-钙粘素 \| E-钙粘蛋白 \| 钙粘蛋白 1小鼠单克隆抗体 \| E-钙粘蛋白抗体 \| Anti-CDH1抗体 \| E-Cadherin抗体 \| E-钙粘蛋白小鼠单克隆抗体 \| E-钙粘蛋白小鼠单抗
英文别名	Arc 1 antibody \| CADH1_HUMAN antibody \| Cadherin 1 antibody \| cadherin 1 type 1 E-cadherin antibody \| Cadherin-1 antibody \| Cadherin1 antibody \| CAM 120/80 antibody \| CD 324 antibody \| CD324 antibody \| CD324 antigen antibody \| cdh1 antibody \| CDHE antibod
规格或纯度	Carrier Free, ExactAb™, Azide Free, Validated, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	E-Cadherin
种属反应性	人(Human)
预测反应种属	Gorilla gorilla gorilla
免疫原	Recombinant protein corresponding to Human E-Cadherin
阳性对照	WB: MCF-7 and LNCaP whole cell lysates ICC/IF: MCF-7 cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	AB03/2B8
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

CDH1 Tbio 钙粘蛋白-1(Cadherin-1) (0 活性数据)

点击查看靶蛋白信息： CDH1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: E-Cadherin Mouse mAb (Ab186487)

E-Cadherin Mouse mAb (Ab186487) - Western Blot
All lanes: E-Cadherin Mouse Antibody (Ab186487) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 97 kDa
Observed band size: 109 kDa
Exposure time: 20.6 s

ICC/IF: E-Cadherin Mouse mAb (Ab186487)

E-Cadherin Mouse mAb (Ab186487) - ICC/IF
IF analysis of E-Cadherin (Red) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with E-Cadherin Mouse mAb (Ab186487) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.