PAR650097 (anti-PAR2)

COA

级别和纯度:

≥95%(SDS-PAGE&SEC-HPLC)
见COA

应用:

Animal Model
ELISA
Flow Cytometry
Functional Assay

宿主种属: 人(Human)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Human IgG1
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab177866-100μg	100μg	现货
Ab177866-1mg	1mg	现货
Ab177866-5mg	5mg	现货
Ab177866-10mg	10mg	期货

查看相关系列

点击查看靶蛋白信息: F2RL1

Accession#:P55085 (2) F2RL1 (1) Gene ID:2150 (2) PAR2 (1)

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基本信息

产品名称	PAR650097 (anti-PAR2)
别名	PAR650097（抗 PAR2）
英文别名	Coagulation factor II receptor-like 1 \| G-protein coupled receptor 11 \| PAR-2 \| Thrombin receptor-like 1
规格或纯度	无载体, 重组, ExactAb™, 低内毒素, 无叠氮钠, 已验证, 无动物源, ≥95%(SDS-PAGE&SEC-HPLC), 见COA
宿主种属	人(Human)
特异性	F2RL1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Human IgG1
轻链亚型	lambda
SDS-PAGE	30.2 kDa (Light Chain) & 52.7 kDa (Heavy Chain), under reducing conditions; 232.1 kDa, under non-reducing conditions.
纯化方法	Protein A purified
纯度	>95%
物理外观	Liquid
储存缓冲液	Supplied as a 0.22μm filtered solution in 100mM Pro-Ac, 20mM Arg, pH 5.0
防腐剂	No
浓度	见COA
储存温度	-80℃储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -80℃ 下保存 24 个月。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

F2RL1 Tchem 蛋白酶激活受体 2(Proteinase-activated receptor 2) (0 活性数据)

点击查看靶蛋白信息： F2RL1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: PAR650097 (anti-PAR2) (Ab177866)

PAR650097 (anti-PAR2) (Ab177866) - Flow Cytometry
pLVX-puro-SYMX01-PAR650097-FL cells were stained with PAR650097 (anti-PAR2) (Ab177866) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, the EC50 for this effect is 11.42 nM.

SEC: PAR650097 (anti-PAR2) (Ab177866)

PAR650097 (anti-PAR2) (Ab177866) - SEC
The purity of PAR650097 (anti-PAR2) (Ab177866) is more than 95% verified by HPLC.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0607178	分析证书	Ab177866
ZJ24F0607177	分析证书	Ab177866
ZJ24F0607176	分析证书	Ab177866

引用文献

1. Chackalamannil S, Wang Y, Greenlee WJ, Hu Z, Xia Y, Ahn HS, Boykow G, Hsieh Y, Palamanda J, Agans-Fantuzzi J et al.. (2008) Discovery of a novel, orally active himbacine-based thrombin receptor antagonist (SCH 530348) with potent antiplatelet activity.. J Med Chem, 51 (11): (3061-4). [PMID:18447380]
2. Lee MC, Huang SC. (2008) Proteinase-activated receptor-1 (PAR(1)) and PAR(2) but not PAR(4) mediate contraction in human and guinea-pig gallbladders.. Neurogastroenterol Motil, 20 (4): (385-91). [PMID:18179608]
3. Barry GD, Suen JY, Le GT, Cotterell A, Reid RC, Fairlie DP. (2010) Novel agonists and antagonists for human protease activated receptor 2.. J Med Chem, 53 (20): (7428-40). [PMID:20873792]
4. Takizawa T, Tamiya M, Hara T, Matsumoto J, Saito N, Kanke T, Kawagoe J, Hattori Y. (2005) Abrogation of bronchial eosinophilic inflammation and attenuated eotaxin content in protease-activated receptor 2-deficient mice.. J Pharmacol Sci, 98 (1): (99-102). [PMID:15879675]
5. Khoufache K, LeBouder F, Morello E, Laurent F, Riffault S, Andrade-Gordon P, Boullier S, Rousset P, Vergnolle N, Riteau B. (2009) Protective role for protease-activated receptor-2 against influenza virus pathogenesis via an IFN-gamma-dependent pathway.. J Immunol, 182 (12): (7795-802). [PMID:19494303]
6. Noorbakhsh F, Tsutsui S, Vergnolle N, Boven LA, Shariat N, Vodjgani M, Warren KG, Andrade-Gordon P, Hollenberg MD, Power C. (2006) Proteinase-activated receptor 2 modulates neuroinflammation in experimental autoimmune encephalomyelitis and multiple sclerosis.. J Exp Med, 203 (2): (425-35). [PMID:16476770]
7. Seeliger S, Derian CK, Vergnolle N, Bunnett NW, Nawroth R, Schmelz M, Von Der Weid PY, Buddenkotte J, Sunderkötter C, Metze D et al.. (2003) Proinflammatory role of proteinase-activated receptor-2 in humans and mice during cutaneous inflammation in vivo.. FASEB J, 17 (13): (1871-85). [PMID:14519665]
8. Migliore M, Habrant D, Sasso O, Albani C, Bertozzi SM, Armirotti A, Piomelli D, Scarpelli R. (2016) Potent multitarget FAAH-COX inhibitors: Design and structure-activity relationship studies.. Eur J Med Chem, 109 (216-37). [PMID:26774927]
9. Cheng RKY, Fiez-Vandal C, Schlenker O, Edman K, Aggeler B, Brown DG, Brown GA, Cooke RM, Dumelin CE, Doré AS et al.. (2017) Structural insight into allosteric modulation of protease-activated receptor 2.. Nature, 545 (7652): (112-115). [PMID:28445455]
10. Jiang Y, Yau MK, Lim J, Wu KC, Xu W, Suen JY, Fairlie DP. (2018) A Potent Antagonist of Protease-Activated Receptor 2 That Inhibits Multiple Signaling Functions in Human Cancer Cells.. J Pharmacol Exp Ther, 364 (2): (246-257). [PMID:29263243]
11. LeSarge JC, Thibeault P, Milne M, Ramachandran R, Luyt LG. (2019) High Affinity Fluorescent Probe for Proteinase-Activated Receptor 2 (PAR2).. ACS Med Chem Lett, 10 (7): (1045-1050). [PMID:31312406]
12. O'Brien PJ,Prevost N,Molino M,Hollinger MK,Woolkalis MJ,Woulfe DS,Brass LF. Thrombin responses in human endothelial cells. Contributions from receptors other than PAR1 include the transactivation of PAR2 by thrombin-cleaved PAR1.. J Biol Chem, [PMID:10788464]
13. Boitano S, Flynn AN, Schulz SM, Hoffman J, Price TJ, Vagner J.. (2011) Potent agonists of the protease activated receptor 2 (PAR2).. J Med Chem, 54 (5): (1308-1313). [PMID:21294569] [10.1021/jm1013049]
14. Gandhi DM, Majewski MW, Rosas R, Kentala K, Foster TJ, Greve E, Dockendorff C.. (2018) Characterization of Protease-Activated Receptor (PAR) ligands: Parmodulins are reversible allosteric inhibitors of PAR1-driven calcium mobilization in endothelial cells.. Bioorg Med Chem, 26 (9): (2514-2529). [PMID:29685684] [10.1016/j.bmc.2018.04.016]
15. Gandhi DM, Rosas R, Greve E, Kentala K, D-R Diby N, Snyder VA, Stephans A, Yeung THW, Subramaniam S, DiMilo E, Kurtenbach KE, Arnold LA, Weiler H, Dockendorff C.. (2019) The parmodulin NRD-21 is an allosteric inhibitor of PAR1 Gq signaling with improved anti-inflammatory activity and stability.. Bioorg Med Chem, 27 (17): (3788-3796). [PMID:31320211] [10.1016/j.bmc.2019.06.043]
16. Nystedt, S S, Emilsson, K K, Larsson, A K AK, Strömbeck, B B and Sundelin, J J.. (1995) Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor 2.. European journal of biochemistry, (15): [PMID:7556175]
17. Bohm, S K SK and 10 more authors.. (1996) Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2.. The Biochemical journal, (15): [PMID:8615752]
18. Kahn, M M and 8 more authors.. (1996) Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene cluster.. Molecular medicine (Cambridge, Mass.), [PMID:8784787]
19. Molino, M M and 9 more authors.. (1997) Interactions of mast cell tryptase with thrombin receptors and PAR-2.. The Journal of biological chemistry, (14): [PMID:9020112]
20. DeFea, K A KA and 5 more authors.. (2000) beta-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2.. The Journal of cell biology, (20): [PMID:10725339]
21. Takeuchi, T T and 5 more authors.. (2000) Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates.. The Journal of biological chemistry, (25): [PMID:10831593]
22. Kanke, T T and 6 more authors.. (2001) Proteinase-activated receptor-2-mediated activation of stress-activated protein kinases and inhibitory kappa B kinases in NCTC 2544 keratinocytes.. The Journal of biological chemistry, (24): [PMID:11413129]
23. Sun, G G, Stacey, M A MA, Schmidt, M M, Mori, L L and Mattoli, S S.. (2001) Interaction of mite allergens Der p3 and Der p9 with protease-activated receptor-2 expressed by lung epithelial cells.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11441110]
24. Lourbakos, A A and 7 more authors.. (2001) Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion.. Infection and immunity, [PMID:11447194]
25. Miike, S S, McWilliam, A S AS and Kita, H H.. (2001) Trypsin induces activation and inflammatory mediator release from human eosinophils through protease-activated receptor-2.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11714832]
26. Compton, Steven J SJ, Sandhu, Sabrina S, Wijesuriya, Suranga J SJ and Hollenberg, Morley D MD.. (2002) Glycosylation of human proteinase-activated receptor-2 (hPAR2): role in cell surface expression and signalling.. The Biochemical journal, (1): [PMID:12171601]
27. Bolton, Sarah J SJ, McNulty, Clare A CA, Thomas, Rebecca J RJ, Hewitt, Colin R A CR and Wardlaw, Andrew J AJ.. (2003) Expression of and functional responses to protease-activated receptors on human eosinophils.. Journal of leukocyte biology, [PMID:12832443]
28. Shpacovitch, V M VM and 13 more authors.. (2004) Agonists of proteinase-activated receptor-2 modulate human neutrophil cytokine secretion, expression of cell adhesion molecules, and migration within 3-D collagen lattices.. Journal of leukocyte biology, [PMID:15155775]
29. Jacob, Claire C and 5 more authors.. (2005) c-Cbl mediates ubiquitination, degradation, and down-regulation of human protease-activated receptor 2.. The Journal of biological chemistry, (22): [PMID:15708858]
30. Hansen, Kristina K KK and 8 more authors.. (2005) A major role for proteolytic activity and proteinase-activated receptor-2 in the pathogenesis of infectious colitis.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:15919826]
31. Feistritzer, C C, Lenta, R R and Riewald, M M.. (2005) Protease-activated receptors-1 and -2 can mediate endothelial barrier protection: role in factor Xa signaling.. Journal of thrombosis and haemostasis : JTH, [PMID:16359518]
32. Luo, Weibo W, Wang, Yingfei Y, Hanck, Theodor T, Stricker, Rolf R and Reiser, Georg G.. (2006) Jab1, a novel protease-activated receptor-2 (PAR-2)-interacting protein, is involved in PAR-2-induced activation of activator protein-1.. The Journal of biological chemistry, (24): [PMID:16410250]
33. Csernok, Elena E and 8 more authors.. (2006) Wegener autoantigen induces maturation of dendritic cells and licenses them for Th1 priming via the protease-activated receptor-2 pathway.. Blood, (1): [PMID:16478888]
34. Winter, Michael C MC, Shasby, Sandra S SS, Ries, Dana R DR and Shasby, D Michael DM.. (2006) PAR2 activation interrupts E-cadherin adhesion and compromises the airway epithelial barrier: protective effect of beta-agonists.. American journal of physiology. Lung cellular and molecular physiology, [PMID:16714334]
35. Chiu, Li-Li LL, Perng, Diahn-Warng DW, Yu, Chia-Hsien CH, Su, Song-Nan SN and Chow, Lu-Ping LP.. (2007) Mold allergen, pen C 13, induces IL-8 expression in human airway epithelial cells by activating protease-activated receptor 1 and 2.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:17404307]
36. Zoudilova, Maria M and 5 more authors.. (2007) Beta-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2.. The Journal of biological chemistry, (13): [PMID:17500066]
37. Luo, Weibo W, Wang, Yingfei Y and Reiser, Georg G.. (2007) p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of protease-activated receptor-2 by influence on receptor trafficking.. The Journal of biological chemistry, (12): [PMID:17693410]
38. Goon Goh, Fui F and 5 more authors.. (2008) G-protein-dependent and -independent pathways regulate proteinase-activated receptor-2 mediated p65 NFkappaB serine 536 phosphorylation in human keratinocytes.. Cellular signalling, [PMID:18424071]
39. Feld, Micha M and 7 more authors.. (2008) Agonists of proteinase-activated receptor-2 enhance IFN-gamma-inducible effects on human monocytes: role in influenza A infection.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:18453611]
40. Hong, Jeong Hee JH and 7 more authors.. (2008) Chitinase activates protease-activated receptor-2 in human airway epithelial cells.. American journal of respiratory cell and molecular biology, [PMID:18474671]
41. Rallabhandi, Prasad P and 7 more authors.. (2008) Analysis of proteinase-activated receptor 2 and TLR4 signal transduction: a novel paradigm for receptor cooperativity.. The Journal of biological chemistry, (5): [PMID:18622013]
42. Hasdemir, Burcu B, Murphy, Jane E JE, Cottrell, Graeme S GS and Bunnett, Nigel W NW.. (2009) Endosomal deubiquitinating enzymes control ubiquitination and down-regulation of protease-activated receptor 2.. The Journal of biological chemistry, (9): [PMID:19684015]
43. McIntosh, Kathryn K and 6 more authors.. (2010) Proteinase-activated receptor-2 mediated inhibition of TNFalpha-stimulated JNK activation - A novel paradigm for G(q/11) linked GPCRs.. Cellular signalling, [PMID:19781631]
44. Ricks, Tiffany K TK and Trejo, JoAnn J.. (2009) Phosphorylation of protease-activated receptor-2 differentially regulates desensitization and internalization.. The Journal of biological chemistry, (4): [PMID:19815543]
45. Matsuwaki, Yoshinori Y and 10 more authors.. (2009) Recognition of fungal protease activities induces cellular activation and eosinophil-derived neurotoxin release in human eosinophils.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:19864598]
46. Nhu, Q M QM and 7 more authors.. (2010) Novel signaling interactions between proteinase-activated receptor 2 and Toll-like receptors in vitro and in vivo.. Mucosal immunology, [PMID:19865078]
47. Holzhausen, M M and 5 more authors.. (2010) Protease-activated receptor-2 (PAR(2)) in human periodontitis.. Journal of dental research, [PMID:20530726]
48. Bae, Jong-Sup JS, Yang, Likui L and Rezaie, Alireza R AR.. (2010) Factor X/Xa elicits protective signaling responses in endothelial cells directly via PAR-2 and indirectly via endothelial protein C receptor-dependent recruitment of PAR-1.. The Journal of biological chemistry, (5): [PMID:20826780]
49. Shpacovitch, Victoria M VM and 10 more authors.. (2011) Role of proteinase-activated receptor-2 in anti-bacterial and immunomodulatory effects of interferon-γ on human neutrophils and monocytes.. Immunology, [PMID:21501162]
50. Botham, Andrew A and 5 more authors.. (2011) Palmitoylation of human proteinase-activated receptor-2 differentially regulates receptor-triggered ERK1/2 activation, calcium signalling and endocytosis.. The Biochemical journal, (1): [PMID:21627585]
51. Kuckleburg, Christopher J CJ and Newman, Peter J PJ.. (2013) Neutrophil proteinase 3 acts on protease-activated receptor-2 to enhance vascular endothelial cell barrier function.. Arteriosclerosis, thrombosis, and vascular biology, [PMID:23202369]

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PAR650097 (anti-PAR2)

库存信息

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库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器