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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab176386-10μg |
10μg |
现货 ![]() |
| |
| Ab176386-50μg |
50μg |
现货 ![]() |
| |
| Ab176386-100μg |
100μg |
期货 ![]() |
| |
| Ab176386-1mg |
1mg |
期货 ![]() |
|
| 产品名称 | PLGF Mouse mAb |
|---|---|
| 别名 | 胎盘生长因子抗体 | 抗PLGF抗体 | PLGF 小鼠单克隆抗体 | PLGF 小鼠单抗 |
| 英文别名 | D12S1900 | PGF | PGFL | placenta growth factor | placental growth factor | placental growth factor, vascular endothelial growth factor-related protein | PlGF | PlGF-2 | PLGFplacental growth factor-like | SHGC-10760 | D12S1900 antibody | Pgf antibody | PGF |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | PGF |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant Human PLGF Protein, C-His tag |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | 7G1-2 |
| Format | Whole IgG |
| 亚型 | Mouse IgG1 |
| 轻链亚型 | kappa |
| SDS-PAGE | 25.8 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 175.6 kDa, under non-reducing conditions. |
| 纯化方法 | Affinity chromatography |
| 配对抗体 | [Ab176388] PLGF Mouse mAb as Capture or Detection antibody in ELISA, CLIA, LF, GICA, FIA |
| 物理外观 | Liquid |
| 储存缓冲液 | 0.01M PBS, pH7.4 |
| 防腐剂 | No |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 37°C 下短期保存(7 天) 4°C 下短期保存(30 天)。在 -20±5°C 下长期储存(36 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Sandwich ELISA - PLGF protein standard curve
Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176386) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176388) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence
·Capture antibody: PLGF Mouse mAb (Ab176386)
·Detection antibody: PLGF Mouse mAb (Ab176388)
Sandwich ELISA - PLGF protein standard curve
Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176388) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176386) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence
·Capture antibody: PLGF Mouse mAb (Ab176388)
·Detection antibody: PLGF Mouse mAb (Ab176386)
Binding ELISA - PLGF Mouse mAb (Ab176386)
Immobilized Recombinant Human PLGF Protein at 1.0 μg/mL can bind PLGF Mouse mAb (Ab176386) with the EC₅₀ of 21.40 ng/mL.
| 应用名称 | 稀释比例 |
|---|---|
| CLIA |
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
|
| FLFIA |
Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab176386 | |
| 分析证书 | Ab176386 | |
| 分析证书 | Ab176386 |
¥375.92
| 1. Maglione, D D, Guerriero, V V, Viglietto, G G, Delli-Bovi, P P and Persico, M G MG.. (1991) Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1924389] |
| 2. Maglione, D D and 9 more authors.. (1993) Two alternative mRNAs coding for the angiogenic factor, placenta growth factor (PlGF), are transcribed from a single gene of chromosome 14.. Oncogene, [PMID:7681160] |
| 3. Park, J E JE, Chen, H H HH, Winer, J J, Houck, K A KA and Ferrara, N N.. (1994) Placenta growth factor. Potentiation of vascular endothelial growth factor bioactivity, in vitro and in vivo, and high affinity binding to Flt-1 but not to Flk-1/KDR.. The Journal of biological chemistry, (14): [PMID:7929268] |
| 4. Hauser, S S and Weich, H A HA.. (1993) A heparin-binding form of placenta growth factor (PlGF-2) is expressed in human umbilical vein endothelial cells and in placenta.. Growth factors (Chur, Switzerland), [PMID:8148155] |
| 5. Cao, Y Y, Ji, W R WR, Qi, P P, Rosin, A A and Cao, Y Y.. (1997) Placenta growth factor: identification and characterization of a novel isoform generated by RNA alternative splicing.. Biochemical and biophysical research communications, (27): [PMID:9207183] |
| 6. Iyer, S S and 7 more authors.. (2001) The crystal structure of human placenta growth factor-1 (PlGF-1), an angiogenic protein, at 2.0 A resolution.. The Journal of biological chemistry, (13): [PMID:11069911] |
| 7. Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6): [PMID:12508121] |
| 8. Yang, Wen W, Ahn, Henry H, Hinrichs, Monica M, Torry, Ronald J RJ and Torry, Donald S DS.. (2003) Evidence of a novel isoform of placenta growth factor (PlGF-4) expressed in human trophoblast and endothelial cells.. Journal of reproductive immunology, [PMID:14568677] |
| 9. Rolny, Charlotte C and 26 more authors.. (2011) HRG inhibits tumor growth and metastasis by inducing macrophage polarization and vessel normalization through downregulation of PlGF.. Cancer cell, (18): [PMID:21215706] |
| 10. Browning, David J DJ, Kaiser, Peter K PK, Rosenfeld, Philip J PJ and Stewart, Michael W MW.. (2012) Aflibercept for age-related macular degeneration: a game-changer or quiet addition?. American journal of ophthalmology, [PMID:22813448] |