RBP4 Mouse mAb

COA

级别和纯度:

见COA

应用:

Capture Antibody
CLIA
ELISA
FIA
FLFIA
GICA
LF

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: 9D11-8
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab176165-10μg	10μg	现货
Ab176165-50μg	50μg	现货
Ab176165-100μg	100μg	现货
Ab176165-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: RBP4

Accession#:P02753 (5) Gene ID:5950 (4) RBP4 (5)

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基本信息

产品名称	RBP4 Mouse mAb
别名	RBP4 小鼠 mAb
英文别名	Plasma retinol-binding protein \| PRBP \| RBP
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	RBP4
种属反应性	人(Human)
免疫原	Recombinant Human RBP4 Protein, C-His tag
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	9D11-8
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	25.9 kDa (Light Chain) & 51.3 kDa (Heavy Chain), under reducing conditions; 172.8 kDa, under non-reducing conditions.
纯化方法	Affinity chromatography
配对抗体	[Ab176170] RBP4 Mouse mAb as Detection antibody in ELISA, CLIA, LF, GICA, FIA
物理外观	Liquid
储存缓冲液	10mM PBS, pH7.4
防腐剂	No
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	2 - 8°C短期储存（30 天）。-20°C下长期保存（36个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

RBP4 Tchem 视黄醇结合蛋白 4(Retinol-binding protein 4) (0 活性数据)

点击查看靶蛋白信息： RBP4

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Sandwich ELISA - RBP4 Mouse mAb Pair (Ab176165 & Ab176170)

Sandwich ELISA - RBP4 protein standard curve
Human RBP4 protein was serially diluted 2-fold and captured by RBP4 Mouse mAb (Ab176165) coated on a Clear Polystyrene Microplate (W291037). RBP4 Mouse mAb (Ab176170) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Relevance of detection results between aladdin Antibody Pair (Ab176165 & Ab176170) and R&D in CLIA

Relevance of detection results between aladdin Antibody Pair (Ab176165 & Ab176170) and R&D in CLIA
·Capture antibody: RBP4 Mouse mAb (Ab176165)
·Detection antibody: RBP4 Mouse mAb (Ab176170)

Binding ELISA - RBP4 Mouse mAb (Ab176165)

Binding ELISA - RBP4 Mouse mAb (Ab176165)
Immobilized Recombinant Human RBP4 Protein at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176165) with the EC₅₀ of 49.39 ng/mL.

应用

应用名称	稀释比例
CLIA	20µg/2mg magnetic beads
FLFIA	Fluorescent beads/Antibody =10/1

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1011879	分析证书	Ab176165
ZJ23F1101567	分析证书	Ab176165
ZJ23F1101568	分析证书	Ab176165

可替换产品

引用文献

1. Campos-Sandoval JA, Redondo C, Kinsella GK, Pal A, Jones G, Eyre GS, Hirst SC, Findlay JB.. (2011) Fenretinide derivatives act as disrupters of interactions of serum retinol binding protein (sRBP) with transthyretin and the sRBP receptor.. J Med Chem, 54 (13): (4378-4387). [PMID:21591606] [10.1021/jm200256g]
2. Cioffi CL, Dobri N, Freeman EE, Conlon MP, Chen P, Stafford DG, Schwarz DM, Golden KC, Zhu L, Kitchen DB, Barnes KD, Racz B, Qin Q, Michelotti E, Cywin CL, Martin WH, Pearson PG, Johnson G, Petrukhin K.. (2014) Design, synthesis, and evaluation of nonretinoid retinol binding protein 4 antagonists for the potential treatment of atrophic age-related macular degeneration and Stargardt disease.. J Med Chem, 57 (18): (7731-7757). [PMID:25210858] [10.1021/jm5010013]
3. Cioffi CL, Racz B, Freeman EE, Conlon MP, Chen P, Stafford DG, Schwarz DM, Zhu L, Kitchen DB, Barnes KD, Dobri N, Michelotti E, Cywin CL, Martin WH, Pearson PG, Johnson G, Petrukhin K.. (2015) Bicyclic [3.3.0]-Octahydrocyclopenta[c]pyrrolo Antagonists of Retinol Binding Protein 4: Potential Treatment of Atrophic Age-Related Macular Degeneration and Stargardt Disease.. J Med Chem, 58 (15): (5863-5888). [PMID:26181715] [10.1021/acs.jmedchem.5b00423]
4. Cioffi CL, Racz B, Varadi A, Freeman EE, Conlon MP, Chen P, Zhu L, Kitchen DB, Barnes KD, Martin WH, Pearson PG, Johnson G, Blaner WS, Petrukhin K.. (2019) Design, Synthesis, and Preclinical Efficacy of Novel Nonretinoid Antagonists of Retinol-Binding Protein 4 in the Mouse Model of Hepatic Steatosis.. J Med Chem, 62 (11): (5470-5500). [PMID:31079449] [10.1021/acs.jmedchem.9b00352]
5. Cioffi CL,Muthuraman P,Raja A,Varadi A,Racz B,Petrukhin K. (2020) Discovery of Bispecific Antagonists of Retinol Binding Protein 4 That Stabilize Transthyretin Tetramers: Scaffolding Hopping, Optimization, and Preclinical Pharmacological Evaluation as a Potential Therapy for Two Common Age-Related Comorbidities.. J Med Chem, 63 (19): (11054-11084). [PMID:32878437] [10.1021/acs.jmedchem.0c00996]
6. Rask, L L, Anundi, H H and Peterson, P A PA.. (1979) The primary structure of the human retinol-binding protein.. FEBS letters, (1): [PMID:573217]
7. Monaco, H L HL and Zanotti, G G.. (1992) Three-dimensional structure and active site of three hydrophobic molecule-binding proteins with significant amino acid sequence similarity.. Biopolymers, [PMID:1623143]
8. Cowan, S W SW, Newcomer, M E ME and Jones, T A TA.. (1990) Crystallographic refinement of human serum retinol binding protein at 2A resolution.. Proteins, [PMID:2217163]
9. Rask, L L, Anundi, H H, Fohlman, J J and Peterson, P A PA.. (1987) The complete amino acid sequence of human serum retinol-binding protein.. Upsala journal of medical sciences, [PMID:2444024]
10. D'Onofrio, C C, Colantuoni, V V and Cortese, R R.. (1985) Structure and cell-specific expression of a cloned human retinol binding protein gene: the 5'-flanking region contains hepatoma specific transcriptional signals.. The EMBO journal, [PMID:2998779]
11. Colantuoni, V V and 6 more authors.. (1983) Cloning and sequencing of a full length cDNA coding for human retinol-binding protein.. Nucleic acids research, (25): [PMID:6316270]
12. Newcomer, M E ME and 6 more authors.. (1984) The three-dimensional structure of retinol-binding protein.. The EMBO journal, [PMID:6540172]
13. Rask, L L and 6 more authors.. (1981) Structural and functional studies of vitamin A-binding proteins.. Annals of the New York Academy of Sciences, (27): [PMID:6942701]
14. Jaconi, S S, Rose, K K, Hughes, G J GJ, Saurat, J H JH and Siegenthaler, G G.. (1995) Characterization of two post-translationally processed forms of human serum retinol-binding protein: altered ratios in chronic renal failure.. Journal of lipid research, [PMID:7666002]
15. Monaco, H L HL, Rizzi, M M and Coda, A A.. (1995) Structure of a complex of two plasma proteins: transthyretin and retinol-binding protein.. Science (New York, N.Y.), (19): [PMID:7754382]
16. Seeliger, M W MW and 7 more authors.. (1999) Phenotype in retinol deficiency due to a hereditary defect in retinol binding protein synthesis.. Investigative ophthalmology & visual science, [PMID:9888420]
17. Naylor, H M HM and Newcomer, M E ME.. (1999) The structure of human retinol-binding protein (RBP) with its carrier protein transthyretin reveals an interaction with the carboxy terminus of RBP.. Biochemistry, (2): [PMID:10052934]
18. Biesalski, H K HK and 9 more authors.. (1999) Biochemical but not clinical vitamin A deficiency results from mutations in the gene for retinol binding protein.. The American journal of clinical nutrition, [PMID:10232633]
19. Kiernan, Urban A UA, Tubbs, Kemmons A KA, Nedelkov, Dobrin D, Niederkofler, Eric E EE and Nelson, Randall W RW.. (2002) Comparative phenotypic analyses of human plasma and urinary retinol binding protein using mass spectrometric immunoassay.. Biochemical and biophysical research communications, (20): [PMID:12237133]
20. Kiernan, Urban A UA and 5 more authors.. Comparative urine protein phenotyping using mass spectrometric immunoassay.. Journal of proteome research, [PMID:12716133]
21. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054]
22. Breustedt, Daniel A DA, Schönfeld, Dorian L DL and Skerra, Arne A.. (2006) Comparative ligand-binding analysis of ten human lipocalins.. Biochimica et biophysica acta, [PMID:16461020]
23. Zanotti, Giuseppe G and 8 more authors.. (2008) Structural and mutational analyses of protein-protein interactions between transthyretin and retinol-binding protein.. The FEBS journal, [PMID:19021760]
24. Berry, Daniel C DC, O'Byrne, Sheila M SM, Vreeland, Amanda C AC, Blaner, William S WS and Noy, Noa N.. (2012) Cross talk between signaling and vitamin A transport by the retinol-binding protein receptor STRA6.. Molecular and cellular biology, [PMID:22665496]
25. Cukras, Catherine C and 11 more authors.. (2012) Exome analysis identified a novel mutation in the RBP4 gene in a consanguineous pedigree with retinal dystrophy and developmental abnormalities.. PloS one, [PMID:23189188]
26. Chou, Christopher M CM and 7 more authors.. (2015) Biochemical Basis for Dominant Inheritance, Variable Penetrance, and Maternal Effects in RBP4 Congenital Eye Disease.. Cell, (23): [PMID:25910211]

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分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

RBP4 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器