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Vimentin Mouse mAb

    应用:
  • Flow Cytometry
  • IF/ICC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 小鼠(Mouse)
  • 亚型: Mouse IgG2a
  • 克隆号: AB10/4F11
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab156188-10μl
10μl 现货 Stock Image
Ab156188-50μl
50μL 现货 Stock Image
Ab156188-100μl
100μL 现货 Stock Image
Ab156188-1ml
1ml 期货 Stock Image

基本信息

产品名称 Vimentin Mouse mAb
别名 波形蛋白抗体 | 波形纤维蛋白 | Anti-Vimentin抗体 | 波形蛋白小鼠 mAb
英文别名 CTRCT30 antibody | Epididymis luminal protein 113 antibody | FLJ36605 antibody | HEL113 antibody | VIM antibody | VIME_HUMAN antibody | Vimentin antibody | Anti-Vimentin Antibody | Vimentin Monoclonal Antibody | Anti-Human VIM/Vimentin Antibody | Mouse an
规格或纯度 ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
宿主种属 小鼠(Mouse)
特异性 Vimentin
种属反应性 人(Human),小鼠(Mouse)
免疫原 Recombinant full length protein corresponding to human Vimentin
阳性对照 WB: HeLa, HEK293, A549, Jurkat and NIH/3T3 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 AB10/4F11
Format Whole IgG
亚型 IgG2a
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 PBS, pH 7.2
防腐剂 No
浓度 1.0 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 下短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

VIM Tbio 波形蛋白(Vimentin) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Vimentin Mouse mAb (Ab156188) - Western Blot
All lanes: Vimentin Antibody (Ab156188) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 53.7 kDa
Observed band size: 54.9 kDa
Exposure time: 26.9 s 

Vimentin Mouse mAb (Ab156188) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab156188) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.

Vimentin Mouse mAb (Ab156188) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab156188) at 2 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
Flow Cytometry

0.016-2.0μg/mL

IF/ICC

2 μg/mL

WB

1/2,000-1/20,000 ; Predicted molecular weight: 54 kDa

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找到4个结果

批号(Lot Number) 证书类型 货号
ZJ25F0521478 分析证书 Ab156188
ZJ24F1011911 分析证书 Ab156188
ZJ23F1101670 分析证书 Ab156188
ZJ23F1101669 分析证书 Ab156188

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