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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab156188-10μl |
10μl |
现货 ![]() |
| |
| Ab156188-50μl |
50μL |
现货 ![]() |
| |
| Ab156188-100μl |
100μL |
现货 ![]() |
| |
| Ab156188-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Vimentin Mouse mAb |
|---|---|
| 别名 | 波形蛋白抗体 | 波形纤维蛋白 | Anti-Vimentin抗体 | 波形蛋白小鼠 mAb |
| 英文别名 | CTRCT30 antibody | Epididymis luminal protein 113 antibody | FLJ36605 antibody | HEL113 antibody | VIM antibody | VIME_HUMAN antibody | Vimentin antibody | Anti-Vimentin Antibody | Vimentin Monoclonal Antibody | Anti-Human VIM/Vimentin Antibody | Mouse an |
| 规格或纯度 | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Vimentin |
| 种属反应性 | 人(Human),小鼠(Mouse) |
| 免疫原 | Recombinant full length protein corresponding to human Vimentin |
| 阳性对照 | WB: HeLa, HEK293, A549, Jurkat and NIH/3T3 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | AB10/4F11 |
| Format | Whole IgG |
| 亚型 | IgG2a |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 防腐剂 | No |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 下短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Vimentin Mouse mAb (Ab156188) - Western Blot
All lanes: Vimentin Antibody (Ab156188) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 54.9 kDa
Exposure time: 26.9 s
Vimentin Mouse mAb (Ab156188) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab156188) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.
Vimentin Mouse mAb (Ab156188) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab156188) at 2 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| Flow Cytometry |
0.016-2.0μg/mL
|
| IF/ICC | 2 μg/mL |
| WB | 1/2,000-1/20,000 ; Predicted molecular weight: 54 kDa |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab156188 | |
| 分析证书 | Ab156188 | |
| 分析证书 | Ab156188 | |
| 分析证书 | Ab156188 |
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