基本信息

产品名称	UBE2Z Mouse mAb
别名	UBE2Z抗体 \| UBE2Z 小鼠 mAb
英文别名	FLJ13855 antibody \| GC17P044341 antibody \| HOYS7 antibody \| Uba6 specific E2 conjugating enzyme 1antibody \| UBA6 specific enzyme E2 antibody \| Uba6-specific E2 conjugating enzyme 1 antibody \| UBE2Z antibody \| UBE2Z_HUMAN antibody \| Ubiquitin carrier prote
规格或纯度	Carrier Free, ExactAb™, Validated, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	UBE2Z
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant protein of human UBE2Z (AA 91-340).
阳性对照	WB: MCF7, Hep G2, HeLa, K562, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. IP: MCF7 lysates.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	K52034_14G2
Format	Whole IgG
亚型	Mouse IgG2a
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	150mM PBS, 0.09% Sodium azide, pH 7.4.
防腐剂	0.09% Sodium azide
浓度	1.0 mg/mL
储存温度	-80℃储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 2-8 ℃ 下保存（3 个月）。在 -20 ℃ 下保存（12 个月）。在 -80 ℃ 下保存（36 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

UBE2Z Tbio 泛素结合酶 E2 Z(Ubiquitin-conjugating enzyme E2 Z) (0 活性数据)

点击查看靶蛋白信息： UBE2Z

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: UBE2Z Mouse mAb (Ab133272)

UBE2Z Mouse mAb (Ab133272) - Western Blot
All lanes: UBE2Z Mouse mAb (Ab133272) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 41 kDa
Exposure time: 13.3 s

IP: UBE2Z Mouse mAb (Ab133272)

UBE2Z Mouse mAb (Ab133272) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of UBE2Z Mouse mAb (Ab133272) with MCF7 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. UBE2Z Mouse mAb (Ab133272) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: MCF7 lysateLane 2: UBE2Z immunoprecipitated from MCF7 lysate by UBE2Z Mouse mAb (Ab133272)
Lane 3: The isotype control antibody instead of UBE2Z Mouse mAb (Ab133272) in MCF7 lysate.Result: UBE2Z Mouse mAb (Ab133272) can immunoprecipitate UBE2Z.

IHC: UBE2Z Mouse mAb (Ab133272)

UBE2Z Mouse mAb (Ab133272) - IHC
IHC analysis of testis tissue by UBE2Z Mouse mAb (Ab133272).IHC was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with UBE2Z Mouse mAb (Ab133272) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the cytoplasm and nuclei.