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TTLL12 Mouse mAb

TTLL12 Mouse mAb

级别和纯度:

见COA

应用:

IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Mouse IgG1
克隆号: OTI4B2
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab132973-10μl	10μl	现货
Ab132973-50μl	50μL	现货
Ab132973-100μl	100μL	期货
Ab132973-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: TTLL12

Accession#: Q14166 (1) Gene ID: 23170 (1) TTLL12 (1)

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基本信息

产品名称	TTLL12 Mouse mAb
别名	TTLL12单克隆抗体
英文别名	dJ526I14.2 \| dJ526I14.2 antibody \| FLJ41795 antibody \| KIAA0153 antibody \| TTL12_HUMAN antibody \| TTLL12 antibody \| Tubulin tyrosine ligase like family, member 12 antibody \| Tubulin--tyrosine ligase-like protein 12 antibody
规格或纯度	敲除验证, ExactAb™, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	TTLL12
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant full length protein of human TTLL12 produced in HEK293T.
阳性对照	WB: 293T, HT-29 and Jurkat cell lysates. IHC: Human breast, adenocarcinoma of breast, colon, adenocarcinoma of colon, kidney, liver, carcinoma of liver, ovary, adenocarcinoma of ovary, pancreas, thyroid, endometrium, adenocarcinoma of endometrium, prostat
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3.
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 运输。收货后建议分装。储存于 -20°C 或 -80°C。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

TTLL12 Tbio 微管蛋白-酪氨酸连接酶类蛋白 12(Tubulin--tyrosine ligase-like protein 12) (0 活性数据)

点击查看靶蛋白信息： TTLL12

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - Western Blot
All lanes: TTLL12 Mouse mAb (Ab132973) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 64.8 s

Western Blot: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - Western Blot
Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT) and TTLL12-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with TTLL12 Mouse mAb (Ab132973) (1/500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control.

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IHC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IHC
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using TTLL12 Mouse mAb (Ab132973). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100℃ for 10 min, dilution 1/50)

IF/ICC: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - IF/ICC
Immunofluorescent staining of Hep G2 cells using TTLL12 Mouse mAb (Ab132973).

Flow: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - Flow
HEK293T cells transfected with either pCMV6-ENTRY TTLL12 (Red) or empty vector control plasmid (Blue) were immunostained with TTLL12 Mouse mAb (Ab132973) (dilution 1/1000), and then analyzed by flow cytometry.

Flow: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - Flow
Flow cytometric analysis of HeLa cells, using TTLL12 Mouse mAb (Ab132973) (Red) compared to a nonspecific negative control antibody (Blue).

Flow: TTLL12 Mouse mAb (Ab132973)

TTLL12 Mouse mAb (Ab132973) - Flow
Flow cytometric analysis of Jurkat cells, using TTLL12 Mouse mAb (Ab132973) (Red) compared to a nonspecific negative control antibody (Blue).

应用

应用名称	稀释比例
Flow Cytometry	1/100
WB	1/2000
IF/ICC	1/100
IHC	1/50

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ25F0117269	分析证书	Ab132973
ZJ25F0117268	分析证书	Ab132973

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