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Recombinant TRAF2 Antibody

Recombinant TRAF2 Antibody

级别和纯度:

见COA

应用:

IF/ICC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab132148-10μl	10μl	现货
Ab132148-50μl	50μL	现货
Ab132148-100μl	100μL	期货
Ab132148-1ml	1ml	期货

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点击查看靶蛋白信息: TRAF2

Accession#:Q12933 (2) Gene ID:7186 (2) TRAF2 (3) TRAP (3) TRAP3 (1)

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基本信息

产品名称	Recombinant TRAF2 Antibody
别名	TRAF2抗体 \| 肿瘤坏死因子受体相关因子2抗体
英文别名	E3 ubiquitin-protein ligase TRAF2 antibody \| MGC:45012 antibody \| OTTHUMP00000022625 antibody \| OTTHUMP00000064745 antibody \| TNF receptor associated factor 2 antibody \| TNF receptor-associated factor 2 antibody \| TNF receptor-associated protein antibody
规格或纯度	重组, ExactAb™, 已验证, 见COA
生化机理	调节 NF-kappa-B 和 JNK 的活化，在调节细胞存活和凋亡中发挥核心作用。需要 IgM 到 IgG 的正常抗体同型转换。具有 E3 泛素蛋白连接酶活性，可促进目标蛋白（如 BIRC3、RIPK1 和 TICAM1）与 "Lys-63 "连接的泛素化。是多个 E3 泛素蛋白连接酶复合物的重要组成成分，它通过使目标蛋白与其他 E3 泛素连接酶接触，促进目标蛋白的泛素化。通过抑制 BIRC2 和 BIRC3 蛋白的自泛素化和随后的降解来调节它们的水平；这并不依赖于 TRAF2 的 RING 型锌指结构域。
宿主种属	兔(Rabbit)
特异性	TRAF2
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A synthetic peptide derived from human TRAF2 (AA 1-28)
阳性对照	WB: HeLa, Jurkat, Hep G2, MCF7, SH-SY5Y, THP-1, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH7.4
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

TRAF2 Tbio TNF 受体相关因子 2(TNF receptor-associated factor 2) (0 活性数据)

点击查看靶蛋白信息： TRAF2

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Recombinant TRAF2 Antibody (Ab132148)

Recombinant TRAF2 Antibody (Ab132148) - Western Blot
All lanes: Recombinant TRAF2 Antibody (Ab132148) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 54 kDa
Exposure time: 40.0 s

IF/ICC: Recombinant TRAF2 Antibody (Ab132148)

Recombinant TRAF2 Antibody (Ab132148) - IF/ICC
IF analysis of Recombinant TRAF2 Antibody (Ab132148) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant TRAF2 Antibody (Ab132148) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

应用

应用名称	稀释比例
WB	1/500-1/1000
IHC	1/50 - 1/100
IF/ICC	1/200

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ25F0117332	分析证书	Ab132148
ZJ25F0117331	分析证书	Ab132148

引用文献

1. Chadee DN, Yuasa T, Kyriakis JM. (2002) Direct activation of mitogen-activated protein kinase kinase kinase MEKK1 by the Ste20p homologue GCK and the adapter protein TRAF2.. Mol Cell Biol, 22 (3): (737-49). [PMID:11784851]
2. Yuefeng Huang,Liang Chen,Yi Zhou,Heng Liu,Jueqing Yang,Zhenggang Liu,Chen Wang. (2011-02-11) UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation.. Molecular biology of the cell, 22 ((8)): (1389-1397). [PMID:21307340]
3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
4. Song, H Y HY and Donner, D B DB.. (1995) Association of a RING finger protein with the cytoplasmic domain of the human type-2 tumour necrosis factor receptor.. The Biochemical journal, (1): [PMID:7639698]
5. Rothe, M M, Wong, S C SC, Henzel, W J WJ and Goeddel, D V DV.. (1994) A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor.. Cell, (26): [PMID:8069916]
6. Lee, S Y SY, Park, C G CG and Choi, Y Y.. (1996) T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor necrosis factor receptor-associated factors.. The Journal of experimental medicine, (1): [PMID:8627180]
7. Rothe, M M and 5 more authors.. (1996) I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:8710854]
8. Malinin, N L NL, Boldin, M P MP, Kovalenko, A V AV and Wallach, D D.. (1997) MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1.. Nature, (6): [PMID:9020361]
9. Lee, S Y SY, Lee, S Y SY and Choi, Y Y.. (1997) TRAF-interacting protein (TRIP): a novel component of the tumor necrosis factor receptor (TNFR)- and CD30-TRAF signaling complexes that inhibits TRAF2-mediated NF-kappaB activation.. The Journal of experimental medicine, (7): [PMID:9104814]
10. Hsu, H H and 5 more authors.. (1997) ATAR, a novel tumor necrosis factor receptor family member, signals through TRAF2 and TRAF5.. The Journal of biological chemistry, (23): [PMID:9153189]
11. Arch, R H RH and Thompson, C B CB.. (1998) 4-1BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor kappaB.. Molecular and cellular biology, [PMID:9418902]
12. Kawamata, S S, Hori, T T, Imura, A A, Takaori-Kondo, A A and Uchiyama, T T.. (1998) Activation of OX40 signal transduction pathways leads to tumor necrosis factor receptor-associated factor (TRAF) 2- and TRAF5-mediated NF-kappaB activation.. The Journal of biological chemistry, (6): [PMID:9488716]
13. Gravestein, L A LA and 5 more authors.. (1998) The TNF receptor family member CD27 signals to Jun N-terminal kinase via Traf-2.. European journal of immunology, [PMID:9692890]
14. Thome, M M and 6 more authors.. (1998) Identification of CARDIAK, a RIP-like kinase that associates with caspase-1.. Current biology : CB, (16): [PMID:9705938]
15. Pullen, S S SS and 5 more authors.. (1998) CD40-tumor necrosis factor receptor-associated factor (TRAF) interactions: regulation of CD40 signaling through multiple TRAF binding sites and TRAF hetero-oligomerization.. Biochemistry, (25): [PMID:9718306]
16. Wong, B R BR and 5 more authors.. (1998) The TRAF family of signal transducers mediates NF-kappaB activation by the TRANCE receptor.. The Journal of biological chemistry, (23): [PMID:9774460]
17. Nishitoh, H H and 7 more authors.. (1998) ASK1 is essential for JNK/SAPK activation by TRAF2.. Molecular cell, [PMID:9774977]
18. MacLachlan, T K TK and 5 more authors.. (1998) Binding of CDK9 to TRAF2.. Journal of cellular biochemistry, (15): [PMID:9827693]
19. Kwon, B B and 9 more authors.. (1999) Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.. The Journal of biological chemistry, (5): [PMID:10037686]
20. Park, Y C YC, Burkitt, V V, Villa, A R AR, Tong, L L and Wu, H H.. (1999) Structural basis for self-association and receptor recognition of human TRAF2.. Nature, (8): [PMID:10206649]
21. Baud, V V and 5 more authors.. (1999) Signaling by proinflammatory cytokines: oligomerization of TRAF2 and TRAF6 is sufficient for JNK and IKK activation and target gene induction via an amino-terminal effector domain.. Genes & development, (15): [PMID:10346818]
22. McWhirter, S M SM and 5 more authors.. (1999) Crystallographic analysis of CD40 recognition and signaling by human TRAF2.. Proceedings of the National Academy of Sciences of the United States of America, (20): [PMID:10411888]
23. Ye, X X and 11 more authors.. (1999) TRAF family proteins interact with the common neurotrophin receptor and modulate apoptosis induction.. The Journal of biological chemistry, (15): [PMID:10514511]
24. Fu, C A CA and 5 more authors.. (1999) TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton.. The Journal of biological chemistry, (22): [PMID:10521462]
25. Pomerantz, J L JL and Baltimore, D D.. (1999) NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase.. The EMBO journal, (1): [PMID:10581243]
26. Pype, S S and 9 more authors.. (2000) TTRAP, a novel protein that associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor-associated factors (TRAFs), and that inhibits nuclear factor-kappa B activation.. The Journal of biological chemistry, (16): [PMID:10764746]
27. Eby, M T MT, Jasmin, A A, Kumar, A A, Sharma, K K and Chaudhary, P M PM.. (2000) TAJ, a novel member of the tumor necrosis factor receptor family, activates the c-Jun N-terminal kinase pathway and mediates caspase-independent cell death.. The Journal of biological chemistry, (19): [PMID:10809768]
28. Xia, X Z XZ and 21 more authors.. (2000) TACI is a TRAF-interacting receptor for TALL-1, a tumor necrosis factor family member involved in B cell regulation.. The Journal of experimental medicine, (3): [PMID:10880535]
29. Park, Y C YC and 7 more authors.. (2000) A novel mechanism of TRAF signaling revealed by structural and functional analyses of the TRADD-TRAF2 interaction.. Cell, (23): [PMID:10892748]
30. Kumar, A A, Eby, M T MT, Sinha, S S, Jasmin, A A and Chaudhary, P M PM.. (2001) The ectodermal dysplasia receptor activates the nuclear factor-kappaB, JNK, and cell death pathways and binds to ectodysplasin A.. The Journal of biological chemistry, (26): [PMID:11035039]
31. Yoneda, T T and 6 more authors.. (2001) Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress.. The Journal of biological chemistry, (27): [PMID:11278723]
32. Li, Xiaoming X, Yang, Yili Y and Ashwell, Jonathan D JD.. (2002) TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2.. Nature, (21): [PMID:11907583]
33. Habelhah, Hasem H and 7 more authors.. (2002) Stress-induced decrease in TRAF2 stability is mediated by Siah2.. The EMBO journal, (1): [PMID:12411493]
34. Trompouki, Eirini E and 5 more authors.. (2003) CYLD is a deubiquitinating enzyme that negatively regulates NF-kappaB activation by TNFR family members.. Nature, (14): [PMID:12917689]
35. Kovalenko, Andrew A and 5 more authors.. (2003) The tumour suppressor CYLD negatively regulates NF-kappaB signalling by deubiquitination.. Nature, (14): [PMID:12917691]
36. Gil, Jesús J and 7 more authors.. (2004) TRAF family proteins link PKR with NF-kappa B activation.. Molecular and cellular biology, [PMID:15121867]
37. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053]
38. Zhang, Haifeng H and 5 more authors.. (2004) AIP1/DAB2IP, a novel member of the Ras-GAP family, transduces TRAF2-induced ASK1-JNK activation.. The Journal of biological chemistry, (22): [PMID:15310755]
39. He, Liusheng L, Grammer, Amrie C AC, Wu, Xiaoli X and Lipsky, Peter E PE.. (2004) TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-{kappa}B activation.. The Journal of biological chemistry, (31): [PMID:15383523]
40. van Vliet, Catherine C and 6 more authors.. (2005) Selective regulation of tumor necrosis factor-induced Erk signaling by Src family kinases and the T cell protein tyrosine phosphatase.. Nature immunology, [PMID:15696169]
41. Reiley, William W, Zhang, Minying M, Wu, Xuefeng X, Granger, Erica E and Sun, Shao-Cong SC.. (2005) Regulation of the deubiquitinating enzyme CYLD by IkappaB kinase gamma-dependent phosphorylation.. Molecular and cellular biology, [PMID:15870263]
42. Xu, Liang-Guo LG and 5 more authors.. (2005) VISA is an adapter protein required for virus-triggered IFN-beta signaling.. Molecular cell, (16): [PMID:16153868]
43. Tzimas, Christos C and 5 more authors.. (2006) Human ubiquitin specific protease 31 is a deubiquitinating enzyme implicated in activation of nuclear factor-kappaB.. Cellular signalling, [PMID:16214042]
44. Zhang, Haifeng H and 6 more authors.. (2007) RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation.. The Journal of biological chemistry, (18): [PMID:17389591]
45. Blackwell, Ken K and 5 more authors.. (2009) TRAF2 phosphorylation modulates tumor necrosis factor alpha-induced gene expression and cell resistance to apoptosis.. Molecular and cellular biology, [PMID:18981220]
46. Li, Shitao S, Wang, Lingyan L and Dorf, Martin E ME.. (2009) PKC phosphorylation of TRAF2 mediates IKKalpha/beta recruitment and K63-linked polyubiquitination.. Molecular cell, (16): [PMID:19150425]
47. Yin, Qian Q, Lamothe, Betty B, Darnay, Bryant G BG and Wu, Hao H.. (2009) Structural basis for the lack of E2 interaction in the RING domain of TRAF2.. Biochemistry, (10): [PMID:19810754]
48. Li, Shan S and 11 more authors.. (2010) Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation.. Molecular and cellular biochemistry, [PMID:19937093]
49. Cusick, John K JK, Mustian, Andrea A, Goldberg, Kate K and Reyland, Mary E ME.. (2010) RELT induces cellular death in HEK 293 epithelial cells.. Cellular immunology, [PMID:19969290]
50. Sasai, Miwa M and 6 more authors.. (2010) Direct binding of TRAF2 and TRAF6 to TICAM-1/TRIF adaptor participates in activation of the Toll-like receptor 3/4 pathway.. Molecular immunology, [PMID:20047764]
51. Zhang, Laiqun L, Blackwell, Ken K, Shi, Zhaohui Z and Habelhah, Hasem H.. (2010) The RING domain of TRAF2 plays an essential role in the inhibition of TNFalpha-induced cell death but not in the activation of NF-kappaB.. Journal of molecular biology, (26): [PMID:20064526]
52. Zheng, Chao C, Kabaleeswaran, Venkataraman V, Wang, Yaya Y, Cheng, Genhong G and Wu, Hao H.. (2010) Crystal structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 complexes: affinity, specificity, and regulation.. Molecular cell, (9): [PMID:20385093]
53. Mace, Peter D PD, Smits, Callum C, Vaux, David L DL, Silke, John J and Day, Catherine L CL.. (2010) Asymmetric recruitment of cIAPs by TRAF2.. Journal of molecular biology, (2): [PMID:20447407]
54. Alvarez, Sergio E SE and 11 more authors.. (2010) Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2.. Nature, (24): [PMID:20577214]
55. Scudiero, Ivan I and 5 more authors.. (2011) Alternative splicing of CARMA2/CARD14 transcripts generates protein variants with differential effect on NF-κB activation and endoplasmic reticulum stress-induced cell death.. Journal of cellular physiology, [PMID:21302310]
56. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
57. Cho, J-H JH and 5 more authors.. (2012) Arginine methylation-dependent regulation of ASK1 signaling by PRMT1.. Cell death and differentiation, [PMID:22095282]
58. Kuiken, Hendrik J HJ and 5 more authors.. (2012) Identification of F-box only protein 7 as a negative regulator of NF-kappaB signalling.. Journal of cellular and molecular medicine, [PMID:22212761]
59. Gao, Xiaofei X and 9 more authors.. (2013) NleB, a bacterial effector with glycosyltransferase activity, targets GAPDH function to inhibit NF-κB activation.. Cell host & microbe, (16): [PMID:23332158]
60. Zhou, Alicia Y AY and 6 more authors.. (2013) IKKε-mediated tumorigenesis requires K63-linked polyubiquitination by a cIAP1/cIAP2/TRAF2 E3 ubiquitin ligase complex.. Cell reports, (28): [PMID:23453969]
61. Choi, Seung-Won SW and 10 more authors.. (2018) PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation.. Molecular cell, (7): [PMID:29883609]
62. Saoulli, K K and 10 more authors.. (1998) CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand.. The Journal of experimental medicine, (1): [PMID:9607925]

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Recombinant TRAF2 Antibody

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库存信息

基本信息

产品属性

关联靶点（人）

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应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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引用文献

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